Anti-SNPH antibody (ab69992)
Key features and details
- Rabbit polyclonal to SNPH
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SNPH antibody
See all SNPH primary antibodies -
Description
Rabbit polyclonal to SNPH -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human SNPH aa 80-130 (N terminal). The exact sequence is proprietary.
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Positive control
- Human brain tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 58 kDa).
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Inhibits SNARE complex formation by absorbing free syntaxin-1. -
Tissue specificity
Brain specific. Found in synapses. -
Cellular localization
Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 9751 Human
- Omim: 604942 Human
- SwissProt: O15079 Human
- Unigene: 323833 Human
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Alternative names
- bA314N13.5 antibody
- KIAA0374 antibody
- Snph antibody
see all
Images
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Lane 1 : Anti-SNPH antibody (ab69992) at 2 µg/ml
Lane 2 : Anti-SNPH antibody (ab69992) at 4 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 58 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 120 kDa, 57 kDa, 65 kDa, 70 kDa, 84 kDa. We are unsure as to the identity of these extra bands. -
ab69992 at 5 µg/ml staining SNPH in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunofluorescence of Syntaphilin in Human Brain cells using ab69992 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab69992 has been referenced in 1 publication.
- Feng Y et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923