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RecombinantRabMAb

Recombinant Anti-SNPH antibody [EPR14115(2)] (ab192605)

Reviews (1) Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Anti-SNPH antibody [EPR14115(2)] (ab192605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14115(2)] to SNPH
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SNPH antibody [EPR14115(2)]
    See all SNPH primary antibodies

  • Description

    Rabbit monoclonal [EPR14115(2)] to SNPH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, human hippocampus, rat brain, mouse brain

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

    Immunohistochemical analysis of formalin fixed paraffin embedded human brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

    Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/1000 dilution + Human fetal brain at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/10000 dilution + Human hippocamus at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/5000 dilution + Rat brain at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605)

References (1)

Publishing research using ab192605? Please let us know so that we can cite the reference in this datasheet.

ab192605 has been referenced in 1 publication.

  • Joshi DC  et al. Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell Rep 29:685-696.e5 (2019). PubMed: 31618636

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-SNPH antibody [EPR14115(2)]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hippocampal neurons)
Permeabilization
Yes - 0.1% triton-x100 in PBS
Specification
Hippocampal neurons
Blocking step
1% BSA, 5% FBS in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 22 2017

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