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    snph-antibody-epr141152-ab192605.pdf

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Neuroscience Neurotransmission Secretory Vesicles Regulation
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RecombinantRabMAb

Recombinant Anti-SNPH antibody [EPR14115(2)] (ab192605)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (1)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
  • Anti-SNPH antibody [EPR14115(2)] (ab192605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14115(2)] to SNPH
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-VDAC2 antibody (ab37985)
Protein
Product image
Recombinant Human SNPH protein (ab79146)

View more associated products

Overview

  • Product name

    Anti-SNPH antibody [EPR14115(2)]
    See all SNPH primary antibodies
  • Description

    Rabbit monoclonal [EPR14115(2)] to SNPH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, human hippocampus, rat brain, mouse brain
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14115(2)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Regulation
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs

Associated products

  • Alternative Versions

    • Anti-SNPH antibody [EPR14115(2)] - BSA and Azide free (ab251140)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SNPH protein (ab79146)
  • Related Products

    • Recombinant Human SNPH protein (ab79146)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Inhibits SNARE complex formation by absorbing free syntaxin-1.
  • Tissue specificity

    Brain specific. Found in synapses.
  • Cellular localization

    Membrane. Cell junction > synapse > synaptosome.
  • Target information above from: UniProt accession O15079 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9751 Human
    • Entrez Gene: 241727 Mouse
    • Entrez Gene: 296267 Rat
    • Omim: 604942 Human
    • SwissProt: O15079 Human
    • SwissProt: Q80U23 Mouse
    • SwissProt: B5DF41 Rat
    • Unigene: 323833 Human
    • Unigene: 39312 Mouse
    • Unigene: 133817 Rat
    see all
  • Alternative names

    • bA314N13.5 antibody
    • KIAA0374 antibody
    • Snph antibody
    • SNPH_HUMAN antibody
    • Syntaphilin antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

    Immunohistochemical analysis of formalin fixed paraffin embedded human brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

    Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/1000 dilution + Human fetal brain at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/10000 dilution + Human hippocamus at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/5000 dilution + Rat brain at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (1)

Publishing research using ab192605? Please let us know so that we can cite the reference in this datasheet.

ab192605 has been referenced in 1 publication.

  • Joshi DC  et al. Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell Rep 29:685-696.e5 (2019). PubMed: 31618636

Customer reviews and Q&As

Show All Reviews Q&A
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Immunocytochemistry/ Immunofluorescence abreview for Anti-SNPH antibody [EPR14115(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hippocampal neurons)
Permeabilization
Yes - 0.1% triton-x100 in PBS
Specification
Hippocampal neurons
Blocking step
1% BSA, 5% FBS in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 22 2017

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