Recombinant
RabMAb

Recombinant Anti-SNPH antibody [EPR14115(2)] (ab192605)

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Overview

  • Product name

    Anti-SNPH antibody [EPR14115(2)]
    See all SNPH primary antibodies

  • Description

    Rabbit monoclonal [EPR14115(2)] to SNPH

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SNPH aa 1-100. The exact sequence is proprietary.
    Database link: O15079

  • Positive control

    • Human fetal brain, human hippocampus, rat brain, mouse brain

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab192605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

    Immunohistochemical analysis of formalin fixed paraffin embedded human brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

    Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/1000 dilution + Human fetal brain at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/10000 dilution + Human hippocamus at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)
    Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/5000 dilution + Rat brain at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

References

ab192605 has not yet been referenced specifically in any publications.

Publishing research using ab192605? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-SNPH antibody [EPR14115(2)]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Hippocampal neurons)
Permeabilization
Yes - 0.1% triton-x100 in PBS
Specification
Hippocampal neurons
Blocking step
1% BSA, 5% FBS in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Aug 22 2017

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