Name: Anti-SNPH antibody [EPR14115(2)]
Brand: RabMAb
SKU: ab192605
Rating: 4 (1 reviews)

Product name

Anti-SNPH antibody [EPR14115(2)]
See all SNPH primary antibodies
Description

Rabbit monoclonal [EPR14115(2)] to SNPH
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SNPH aa 1-100. The exact sequence is proprietary.
Database link: O15079
Positive control
- Human fetal brain, human hippocampus, rat brain, mouse brain
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14115(2)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab192605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

Inhibits SNARE complex formation by absorbing free syntaxin-1.
Tissue specificity

Brain specific. Found in synapses.
Cellular localization

Membrane. Cell junction > synapse > synaptosome.
Target information above from: UniProt accession O15079 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 9751 Human
- Entrez Gene: 241727 Mouse
- Entrez Gene: 296267 Rat
- Omim: 604942 Human
- SwissProt: O15079 Human
- SwissProt: Q80U23 Mouse
- SwissProt: B5DF41 Rat
- Unigene: 323833 Human
- Unigene: 39312 Mouse
- Unigene: 133817 Rat
see all
Alternative names
- bA314N13.5 antibody
- KIAA0374 antibody
- Snph antibody
- SNPH_HUMAN antibody
- Syntaphilin antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

Immunohistochemical analysis of formalin fixed paraffin embedded human brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody [EPR14115(2)] (ab192605)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.
Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)

Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/1000 dilution + Human fetal brain at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa
Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)

Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/10000 dilution + Human hippocamus at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa
Western blot - Anti-SNPH antibody [EPR14115(2)] (ab192605)

Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/5000 dilution + Rat brain at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 54 kDa

ab192605 has not yet been referenced specifically in any publications.

Publishing research using ab192605? Please let us know so that we can cite the reference in this datasheet.

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Hippocampal neurons)

Permeabilization

Yes - 0.1% triton-x100 in PBS

Specification

Hippocampal neurons

Blocking step

1% BSA, 5% FBS in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Anti-SNPH antibody [EPR14115(2)] (ab192605)