Recombinant Anti-SNPH antibody [EPR14115(2)] (ab192605)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14115(2)] to SNPH
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SNPH antibody [EPR14115(2)]
See all SNPH primary antibodies -
Description
Rabbit monoclonal [EPR14115(2)] to SNPH -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, human hippocampus, rat brain, mouse brain
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14115(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Inhibits SNARE complex formation by absorbing free syntaxin-1. -
Tissue specificity
Brain specific. Found in synapses. -
Cellular localization
Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 9751 Human
- Entrez Gene: 241727 Mouse
- Entrez Gene: 296267 Rat
- Omim: 604942 Human
- SwissProt: O15079 Human
- SwissProt: Q80U23 Mouse
- SwissProt: B5DF41 Rat
- Unigene: 323833 Human
see all -
Alternative names
- bA314N13.5 antibody
- KIAA0374 antibody
- Snph antibody
see all
Images
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Immunohistochemical analysis of formalin fixed paraffin embedded human brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of formalin fixed paraffin embedded mouse brain labeling SNPH with ab192605 at 1/1000 (1μg/ml) dilution and HRP Polymer to Rabbit/mouse IgG as secondary. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa -
Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/10000 dilution + Human hippocamus tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa -
Anti-SNPH antibody [EPR14115(2)] (ab192605) at 1/5000 dilution + Rat brain at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab192605 has been referenced in 2 publications.
- Huang N et al. Reprogramming an energetic AKT-PAK5 axis boosts axon energy supply and facilitates neuron survival and regeneration after injury and ischemia. Curr Biol 31:3098-3114.e7 (2021). PubMed: 34087103
- Joshi DC et al. Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell Rep 29:685-696.e5 (2019). PubMed: 31618636