Key features and details
- Rabbit polyclonal to SNRNP40
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SNRNP40 antibody
DescriptionRabbit polyclonal to SNRNP40
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
- IHC-P: Human duodenum tissue. ICC/IF: A-431 cells. WB: SNRNP40 overexpressed HEK-293T whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254796 in the following tested applications.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the U5 small nuclear ribonucleoprotein (snRNP) complex. The U5 snRNP is part of the spliceosome, a multiprotein complex that catalyzes the removal of introns from pre-messenger RNAs.
Sequence similaritiesContains 7 WD repeats.
- Information by UniProt
- 38 kDa-splicing factor antibody
- 40K antibody
- FLJ41108 antibody
Paraffin-embedded human duodenum tissue stained for SNRNP40 using ab254796 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-SNRNP40 antibody (ab254796) at 0.4 µg/ml
Lane 1 : NIH/3T3 cell lysate
Lane 2 : NBT-II cell lysate
Lanes 2-4 : Anti-SNRNP40 antibody (ab254796) at 0.4 µg/ml
Lane 1 : DNA Ladder
Lane 2 : Rh30 cells transfected with the control siRNA
Lane 3 : Rh30 cells transfected with the probe #1 siRNA
Lane 4 : Rh30 cells transfected with the probe #2 siRNA
PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling SNRNP40 using ab254796 at 4µg/ml (green) in ICC/IF.
ab254796 has not yet been referenced specifically in any publications.