Overview

  • Product name

    Anti-SNRP70/U1-70K antibody
    See all SNRP70/U1-70K primary antibodies
  • Description

    Rabbit polyclonal to SNRP70/U1-70K
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SNRP70/U1-70K aa 301-350.
    Sequence:

    RERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDR

  • Positive control

    • HepG2 cell lysate
  • General notes

     This product was previously labelled as SNRP70

     

Properties

Applications

Our Abpromise guarantee covers the use of ab51266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance

    SNRP70 is an RNA-binding protein that is a specific component of the U1 small nuclear ribonucleoprotein complex and constitutes the major anti-(U1) RNP autoimmune antigen. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • RNPU1Z antibody
    • Rnulp70 antibody
    • RPU1 antibody
    • Small nuclear ribonucleoprotein 70 (U1) antibody
    • Small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen) antibody
    • Small nuclear ribonucleoprotein 70kDa (U1) antibody
    • Small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen) antibody
    • snRNP70 antibody
    • Snrp 70 antibody
    • SNRP70 antibody
    • U1 70K antibody
    • U1 small nuclear ribonucleoprotein 70 kDa antibody
    • U1 small nuclear ribonucleoprotein polypeptide A antibody
    • U1 snRNP 70 kDa antibody
    • U170K antibody
    • U1AP antibody
    • U1AP1 antibody
    • U1RNP antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SNRP70/U1-70K with ab51266 at 1/100. A Cy3 conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SNRP70/U1-70K. Right - Merge.

  • Anti-SNRP70/U1-70K antibody (ab51266) at 0.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 48,62 kDa
    why is the actual band size different from the predicted?

  • ab51266 (2µg/ml) staining SNRP70/U1-70K in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear stain.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ICC/IF image of ab51266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Zhang Z  et al. Kockdown of OIP5-AS1 expression inhibits proliferation, metastasis and EMT progress in hepatoblastoma cells through up-regulating miR-186a-5p and down-regulating ZEB1. Biomed Pharmacother 101:14-23 (2018). Read more (PubMed: 29475118) »
  • Thompson MG  et al. Co-regulatory activity of hnRNP K and NS1-BP in influenza and human mRNA splicing. Nat Commun 9:2407 (2018). Read more (PubMed: 29921878) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse whole brain tissue lysate, striatum, cortex,)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Loading amount
15 µg
Specification
Mouse whole brain tissue lysate, striatum, cortex,
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 20 2013

Application
Western blot
Sample
Mouse Cell lysate - nuclear (NSC34)
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Loading amount
30 µg
Specification
NSC34
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 10 2011

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