• Product name

    Anti-SNRP70/U1-70K antibody
    See all SNRP70/U1-70K primary antibodies
  • Description

    Rabbit polyclonal to SNRP70/U1-70K
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SNRP70/U1-70K aa 301-350.


  • Positive control

    • HepG2 cell lysate
  • General notes

     This product was previously labelled as SNRP70




Our Abpromise guarantee covers the use of ab51266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • Relevance

    SNRP70 is an RNA-binding protein that is a specific component of the U1 small nuclear ribonucleoprotein complex and constitutes the major anti-(U1) RNP autoimmune antigen. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA.
  • Cellular localization

  • Database links

  • Alternative names

    • RNPU1Z antibody
    • Rnulp70 antibody
    • RPU1 antibody
    • Small nuclear ribonucleoprotein 70 (U1) antibody
    • Small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen) antibody
    • Small nuclear ribonucleoprotein 70kDa (U1) antibody
    • Small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen) antibody
    • snRNP70 antibody
    • Snrp 70 antibody
    • SNRP70 antibody
    • U1 70K antibody
    • U1 small nuclear ribonucleoprotein 70 kDa antibody
    • U1 small nuclear ribonucleoprotein polypeptide A antibody
    • U1 snRNP 70 kDa antibody
    • U170K antibody
    • U1AP antibody
    • U1AP1 antibody
    • U1RNP antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SNRP70/U1-70K with ab51266 at 1/100. A Cy3 conjugated donkey anti-rabbit IgG (1/200) was used as teh secondary antibody. Positive staining shown in the nuclei of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SNRP70/U1-70K. Right - Merge.

  • Anti-SNRP70/U1-70K antibody (ab51266) at 0.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 48,62 kDa
    why is the actual band size different from the predicted?

  • ab51266 (2µg/ml) staining SNRP70/U1-70K in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear stain.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • ICC/IF image of ab51266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Zhang Z  et al. Kockdown of OIP5-AS1 expression inhibits proliferation, metastasis and EMT progress in hepatoblastoma cells through up-regulating miR-186a-5p and down-regulating ZEB1. Biomed Pharmacother 101:14-23 (2018). Read more (PubMed: 29475118) »
  • Thompson MG  et al. Co-regulatory activity of hnRNP K and NS1-BP in influenza and human mRNA splicing. Nat Commun 9:2407 (2018). Read more (PubMed: 29921878) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Mouse Tissue lysate - whole (Mouse whole brain tissue lysate, striatum, cortex,)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12% Bis-Tris Gel, MES running buffer)
Loading amount
15 µg
Mouse whole brain tissue lysate, striatum, cortex,
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 20 2013

Western blot
Mouse Cell lysate - nuclear (NSC34)
Gel Running Conditions
Reduced Denaturing (4-12% Nupage gel)
Loading amount
30 µg
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 10 2011

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