Recombinant
RabMAb

Recombinant Anti-SNRPA1 antibody [EPR7556] (ab128865)

Overview

  • Product name

    Anti-SNRPA1 antibody [EPR7556]
    See all SNRPA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7556] to SNRPA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SNRPA1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat, HUVEC and Human fetal brain lysates; Human colon tissue and Human thyroid gland carcinoma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    Images

    • All lanes : Anti-SNRPA1 antibody [EPR7556] (ab128865) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • ab128865, at 1/50 dilution, staining SNRPA1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • ab128865, at 1/50 dilution, staining SNRPA1 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab128865 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Fibroblast)
    Loading amount
    10 µg
    Specification
    Human Fibroblast
    Gel Running Conditions
    Reduced Denaturing (4-12% bis-Tris Nupage gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up