Overview

  • Product name

    Anti-SNRPA1 antibody [EPR7557]
    See all SNRPA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7557] to SNRPA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SNRPA1 (P09661).

  • Positive control

    • Human colon and squamous lung carcinoma tissues; HeLa, HepG2, Huvec cell lysates; rat and mouse liver lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • All lanes : Anti-SNRPA1 antibody [EPR7557] (ab128937) at 1/1000 dilution

      Lane 1 : Huvec cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Rat liver lysate
      Lane 5 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human colon tissue by immunohistochemistry.
    • ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human squamous lung cell carcinoma tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Soboleva TA  et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . Read more (PubMed: 28234895) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human Fibroblast)
    Loading amount
    10 µg
    Specification
    Human Fibroblast
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Nupage Gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Dec 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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