Name: Anti-SNRPB/SmB antibody
SKU: ab155026

Product name

Anti-SNRPB/SmB antibody
See all SNRPB/SmB primary antibodies
Description

Rabbit polyclonal to SNRPB/SmB
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human SNRPB/SmB aa 1-188.
Positive control
- Hela cells; HeLa nuclear lysate.
General notes

This product was previously labelled as SNRPB

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Positive Controls
- HeLa nuclear extract lysate (ab14655)
- HeLa nuclear extract lysate (ab150036)

Our Abpromise guarantee covers the use of ab155026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/3000. Predicted molecular weight: 25 kDa.
ICC/IF		1/100 - 1/1000.

Function

Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. May have a functional role in the pre-mRNA splicing or in snRNP structure. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Sequence similarities

Belongs to the snRNP SmB/SmN family.
Post-translational
modifications

Methylated. Arg-108 and Arg-112 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localization

Nucleus.
Target information above from: UniProt accession P14678 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6628 Human
- Omim: 182282 Human
- SwissProt: P14678 Human
- Unigene: 83753 Human
Alternative names
- B polypeptide of Sm protein antibody
- COD antibody
- RSMB_HUMAN antibody
- Sm B/B' antibody
- Sm B/Sm B' antibody
- Sm B/SmB' antibody
- Sm protein B/B'' antibody
- Sm-B/B'' antibody
- Small nuclear ribonucleoprotein polypeptide B antibody
- Small nuclear ribonucleoprotein polypeptide B/B' antibody
- Small nuclear ribonucleoprotein polypeptides B and B' antibody
- Small nuclear ribonucleoprotein polypeptides B and B1 antibody
- Small nuclear ribonucleoprotein-associated proteins B and B'' antibody
- SmB antibody
- SmB/B'' antibody
- SmB/Sm B' antibody
- SmB/SmB' antibody
- snRNP B antibody
- snRNP-B antibody
- snRNPB antibody
- SNRPB antibody
- SNRPB1 antibody
see all

Western blot - Anti-SNRPB/SmB antibody (ab155026)

Anti-SNRPB/SmB antibody (ab155026) at 1/1000 dilution + Hela nuclear lysate at 30 µg

Predicted band size: 25 kDa

12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-SNRPB/SmB antibody (ab155026)

Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SNRPB/SmB with ab155026 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.

Western blot protocols
Immunocytochemistry & immunofluorescence protocols

ab155026 has not yet been referenced specifically in any publications.

Publishing research using ab155026? Please let us know so that we can cite the reference in this datasheet.

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Anti-SNRPB/SmB antibody (ab155026)

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