Anti-SNRPB/SmB antibody (ab155026)
Key features and details
- Rabbit polyclonal to SNRPB/SmB
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SNRPB/SmB antibody -
Description
Rabbit polyclonal to SNRPB/SmB -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SNRPB/SmB aa 1-188.
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Positive control
- Hela cells; HeLa nuclear lysate.
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General notes
This product was previously labelled as SNRPB
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab155026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 25 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. May have a functional role in the pre-mRNA splicing or in snRNP structure. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. -
Sequence similarities
Belongs to the snRNP SmB/SmN family. -
Post-translational
modificationsMethylated. Arg-108 and Arg-112 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6628 Human
- Omim: 182282 Human
- SwissProt: P14678 Human
- Unigene: 83753 Human
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Alternative names
- B polypeptide of Sm protein antibody
- COD antibody
- RSMB_HUMAN antibody
see all
Images
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Western blot - Anti-SNRPB/SmB antibody (ab155026)Anti-SNRPB/SmB antibody (ab155026) at 1/1000 dilution + Hela nuclear lysate at 30 µg
Predicted band size: 25 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SNRPB/SmB antibody (ab155026)Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SNRPB/SmB with ab155026 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.
Protocols
References (0)
ab155026 has not yet been referenced specifically in any publications.
