Overview

  • Product name

  • Description

    Rabbit polyclonal to SNRPB/SmB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human SNRPB/SmB aa 71-120 (internal sequence).
    Database link: NP_003082.

  • Positive control

    • 293T cell lysate
  • General notes

     This product was previously labelled as SNRPB

     

Properties

Applications

Our Abpromise guarantee covers the use of ab85534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function

    Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5. May have a functional role in the pre-mRNA splicing or in snRNP structure. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
  • Sequence similarities

    Belongs to the snRNP SmB/SmN family.
  • Post-translational
    modifications

    Methylated. Arg-108 and Arg-112 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B polypeptide of Sm protein antibody
    • COD antibody
    • RSMB_HUMAN antibody
    • Sm B/B' antibody
    • Sm B/Sm B' antibody
    • Sm B/SmB' antibody
    • Sm protein B/B'' antibody
    • Sm-B/B'' antibody
    • Small nuclear ribonucleoprotein polypeptide B antibody
    • Small nuclear ribonucleoprotein polypeptide B/B' antibody
    • Small nuclear ribonucleoprotein polypeptides B and B' antibody
    • Small nuclear ribonucleoprotein polypeptides B and B1 antibody
    • Small nuclear ribonucleoprotein-associated proteins B and B'' antibody
    • SmB antibody
    • SmB/B'' antibody
    • SmB/Sm B' antibody
    • SmB/SmB' antibody
    • snRNP B antibody
    • snRNP-B antibody
    • snRNPB antibody
    • SNRPB antibody
    • SNRPB1 antibody
    see all

Images

  • Anti-SNRPB/SmB antibody (ab85534) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 24 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa, 60 kDa. We are unsure as to the identity of these extra bands.



    Gel concentration 12%

References

ab85534 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85534.
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