Anti-SNRPD1 antibody (ab233115)
Key features and details
- Rabbit polyclonal to SNRPD1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SNRPD1 antibody
See all SNRPD1 primary antibodies -
Description
Rabbit polyclonal to SNRPD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Mouse, Cow, Cynomolgus monkey -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human SNRPD1 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P62314 -
Positive control
- IHC-P: Human liver, prostate, kidney, stomach and cerebrum tissues. WB: HL-60 and Jurkat cell lysates; Rat testis lysate; Pig kidney lysate; Recombinant human SNRPD1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab233115 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 13 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Function
May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through nonspecific electrostatic contacts with RNA. -
Sequence similarities
Belongs to the snRNP core protein family. -
Post-translational
modificationsMethylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 508788 Cow
- Entrez Gene: 101867191 Cynomolgus monkey
- Entrez Gene: 6632 Human
- Entrez Gene: 20641 Mouse
- Entrez Gene: 291794 Rat
- Omim: 601063 Human
- SwissProt: Q3ZC10 Cow
- SwissProt: Q4R5F6 Cynomolgus monkey
see all -
Alternative names
- AA407109 antibody
- AL023031 antibody
- HsT2456 antibody
see all
Images
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Paraffin-embedded human liver tissue stained for SNRPD1 using ab233115 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-SNRPD1 antibody (ab233115) at 2 µg/ml
Lane 1 : Wild type Jurkat cells
Lane 2 : SNRPD1 knockdown Jurkat cells
Predicted band size: 13 kDa -
Paraffin-embedded human prostate tissue stained for SNRPD1 using ab233115 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human kidney tissue stained for SNRPD1 using ab233115 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for SNRPD1 using ab233115 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human cerebrum tissue stained for SNRPD1 using ab233115 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-SNRPD1 antibody (ab233115) at 1 µg/ml + Recombinant human SNRPD1 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 13 kDa -
All lanes : Anti-SNRPD1 antibody (ab233115) at 1 µg/ml
Lane 1 : HL-60 (human promyelocytic leukemia cell line) cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : Rat testis lysate
Lane 4 : Pig kidney lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 13 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab233115 has been referenced in 1 publication.
- Wang H et al. The diagnostic and prognostic significance of small nuclear ribonucleoprotein Sm D1 aberrantly high expression in hepatocellular carcinoma. J Cancer 13:184-201 (2022). PubMed: 34976182