Overview

  • Product name

  • Description

    Rabbit polyclonal to SNRPD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Monkey, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human SNRPD2 aa 1-101.
    Database link: P62316

  • Positive control

    • A549, HeLa, HepG2 and HCT116 whole cell lysate. HeLa cells. Human colon carcinoma tissue.
  • General notes

    Keep as concentrated solution.

    This product was previously labelled as Sm-D2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78% PBS, 20% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-SNRPD2 antibody (ab155030) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size: 14 kDa



    15% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, labeling SNRPD2 using ab155030 at 1/500 dilution (green) and alpha tubulin filaments using a mouse monoclonal antibody at 1/2000 dilution (red).

  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue, labeling SNRPD2 using ab155030 at 1/250 dilution.

References

This product has been referenced in:

  • Gao JL  et al. A conserved intronic U1 snRNP-binding sequence promotes trans-splicing in Drosophila. Genes Dev 29:760-71 (2015). WB . Read more (PubMed: 25838544) »
See 1 Publication for this product

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