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Rabbit polyclonal to SNRPD3
Predicted to work with:
Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
Synthetic peptide corresponding to a region within amino acids 76-126 of Human SNRPD3 (NP_004166.1).
HeLa, 293T and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 14 kDa.
Use at 2-10 µg/mg of lysate.
Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Belongs to the snRNP core protein family.
Methylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
Information by UniProt
Sm D3 antibody
small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
Immunoprecipitation - Anti-SNRPD3 antibody (ab157118)
Immunoprecipitation analysis of whole cell lysate from HeLa cells (1 mg for IP; 20% of IP loaded), labeling SNRPD3 with ab157118 at 6 µg/mg lysate (lane 1), control IgG (lane 2). For blotting immunoprecipitated SNRPD3, ab157118 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western blot - Anti-SNRPD3 antibody (ab157118)
All lanes : Anti-SNRPD3 antibody (ab157118) at 0.4 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 14 kDa Exposure time: 3 minutes
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"