Overview

  • Product name

    Anti-SNRPD3/Sm-D3 antibody
    See all SNRPD3/Sm-D3 primary antibodies
  • Description

    Rabbit polyclonal to SNRPD3/Sm-D3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to Human SNRPD3/Sm-D3 aa 8-121.
    Database link: P62318

  • Positive control

    • RT4, U-251 MG and Human Tonsil cell lysates, Human small intestine tissue
  • General notes

    Previously labelled as SNRPD3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab121129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/100 - 1/250.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
  • Sequence similarities

    Belongs to the snRNP core protein family.
  • Post-translational
    modifications

    Methylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
    Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Sm D3 antibody
    • Sm-D3 antibody
    • small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
    • Small nuclear ribonucleoprotein Sm D3 antibody
    • SmD3 antibody
    • SMD3_HUMAN antibody
    • snRNP core protein D3 antibody
    • SNRPD3 antibody
    see all

Images

  • All lanes : Anti-SNRPD3/Sm-D3 antibody (ab121129) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique.
  • Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
  • ab121129 staining SNRPD3/Sm-D3 in Paraffin-emebedded Human small intestine tissue by Immunohistochemistry. Note: strong nuclear positivity in glandular cells.

References

This product has been referenced in:

  • Hartmann H  et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). Read more (PubMed: 30456350) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121129.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up