Key features and details
- Rabbit polyclonal to SNRPD3/Sm-D3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SNRPD3/Sm-D3 antibody
See all SNRPD3/Sm-D3 primary antibodies
DescriptionRabbit polyclonal to SNRPD3/Sm-D3
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis
Recombinant fragment corresponding to Human SNRPD3/Sm-D3 aa 8-121.
Database link: P62318
- RT4, U-251 MG and Human Tonsil cell lysates, Human small intestine tissue
Previously labelled as SNRPD3.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionAppears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Sequence similaritiesBelongs to the snRNP core protein family.
modificationsMethylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- Sm D3 antibody
- Sm-D3 antibody
- small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
All lanes : Anti-SNRPD3/Sm-D3 antibody (ab121129) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
ab121129 staining SNRPD3/Sm-D3 in Paraffin-emebedded Human small intestine tissue by Immunohistochemistry. Note: strong nuclear positivity in glandular cells.
ab121129 has been referenced in 1 publication.
- Hartmann H et al. Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Sci Alliance 1:e201800070 (2018). PubMed: 30456350