Key features and details
- Rabbit polyclonal to SNRPD3/Sm-D3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SNRPD3/Sm-D3 antibody
See all SNRPD3/Sm-D3 primary antibodies
DescriptionRabbit polyclonal to SNRPD3/Sm-D3
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Orangutan, Zebra finch, Xenopus tropicalis
- HeLa, 293T and Jurkat whole cell lysates.
Previously labelled as SNRPD3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab157118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 14 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionAppears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Sequence similaritiesBelongs to the snRNP core protein family.
modificationsMethylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- Sm D3 antibody
- Sm-D3 antibody
- small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
Immunoprecipitation analysis of whole cell lysate from HeLa cells (1 mg for IP; 20% of IP loaded), labeling SNRPD3/Sm-D3 with ab157118 at 6 µg/mg lysate (lane 1), control IgG (lane 2). For blotting immunoprecipitated SNRPD3/Sm-D3, ab157118 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes : Anti-SNRPD3/Sm-D3 antibody (ab157118) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 14 kDa
Exposure time: 3 minutes
ab157118 has not yet been referenced specifically in any publications.