Key features and details
- Rabbit polyclonal to SNRPD3/Sm-D3
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SNRPD3/Sm-D3 antibody
See all SNRPD3/Sm-D3 primary antibodies
DescriptionRabbit polyclonal to SNRPD3/Sm-D3
Tested applicationsSuitable for: Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Xenopus laevis
Synthetic peptide within Human SNRPD3/Sm-D3 aa 97-126 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P62318
- MCF7, Jurkat cell line lysates; Human lung carcinoma tissue; MCF7 cells.
Previously labelled as SNRPD3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 14 kDa.|
FunctionAppears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
Sequence similaritiesBelongs to the snRNP core protein family.
modificationsMethylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
- Information by UniProt
- Sm D3 antibody
- Sm-D3 antibody
- small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
All lanes : Anti-SNRPD3/Sm-D3 antibody (ab176182) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 14 kDa
Immunohistochemistry analysisof formalin fixed and paraffin embedded Human lung carcinoma labeling SNRPD3/Sm-D3 with ab176182 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MCF7 cells labeling SNRPD3/Sm-D3 with ab176182 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.
ab176182 has not yet been referenced specifically in any publications.