Overview

  • Product name

    Anti-SNRPD3/Sm-D3 antibody
    See all SNRPD3/Sm-D3 primary antibodies
  • Description

    Rabbit polyclonal to SNRPD3/Sm-D3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide within Human SNRPD3/Sm-D3 aa 97-126 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P62318

  • Positive control

    • MCF7, Jurkat cell line lysates; Human lung carcinoma tissue; MCF7 cells.
  • General notes

    Previously labelled as SNRPD3. 

Properties

Applications

Our Abpromise guarantee covers the use of ab176182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 14 kDa.

Target

  • Function

    Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
  • Sequence similarities

    Belongs to the snRNP core protein family.
  • Post-translational
    modifications

    Methylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
    Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Sm D3 antibody
    • Sm-D3 antibody
    • small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
    • Small nuclear ribonucleoprotein Sm D3 antibody
    • SmD3 antibody
    • SMD3_HUMAN antibody
    • snRNP core protein D3 antibody
    • SNRPD3 antibody
    see all

Images

  • All lanes : Anti-SNRPD3/Sm-D3 antibody (ab176182) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 14 kDa

  • Immunohistochemistry analysisof formalin fixed and paraffin embedded Human lung carcinoma labeling SNRPD3/Sm-D3 with ab176182 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of MCF7 cells labeling SNRPD3/Sm-D3 with ab176182 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated Goat-anti-Rabbit secondary antibodies were used for the analysis.

References

ab176182 has not yet been referenced specifically in any publications.

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