Recombinant Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7676] to SNRPD3/Sm-D3
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SNRPD3/Sm-D3 antibody [EPR7676]
See all SNRPD3/Sm-D3 primary antibodies -
Description
Rabbit monoclonal [EPR7676] to SNRPD3/Sm-D3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human SNRPD3/Sm-D3 aa 1-100. The exact sequence is proprietary.
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Positive control
- Jurkat, 293T and Caco-2 cell lysates; 293T cells NIH/3T3 and HEK-293 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7676 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa). |
Target
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Function
Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner. -
Sequence similarities
Belongs to the snRNP core protein family. -
Post-translational
modificationsMethylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
Arg-97 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6634 Human
- Entrez Gene: 67332 Mouse
- GenBank: NP_004166.1 Human
- Omim: 601062 Human
- SwissProt: P62318 Human
- SwissProt: P62320 Mouse
- Unigene: 356549 Human
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Alternative names
- Sm D3 antibody
- Sm-D3 antibody
- small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
see all
Images
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All lanes : Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/5000 dilution (purified)
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/1000 dilution (purified) + NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 14 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab128857 has not yet been referenced specifically in any publications.