Recombinant
RabMAb

Recombinant Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857)

Overview

  • Product name

    Anti-SNRPD3/Sm-D3 antibody [EPR7676]
    See all SNRPD3/Sm-D3 primary antibodies
  • Description

    Rabbit monoclonal [EPR7676] to SNRPD3/Sm-D3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SNRPD3/Sm-D3 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T and Caco-2 cell lysates; 293T cells NIH/3T3 and HEK-293 lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as SNRPD3. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Binds to the downstream cleavage product (DCP) of histone pre-mRNA in a U7 snRNP dependent manner.
    • Sequence similarities

      Belongs to the snRNP core protein family.
    • Post-translational
      modifications

      Methylated on arginine residues by PRMT5 and PRMT7; methylation is required for assembly and biogenesis of snRNPs.
      Arg-97 is dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Sm D3 antibody
      • Sm-D3 antibody
      • small nuclear ribonucleoprotein D3 polypeptide 18kDa antibody
      • Small nuclear ribonucleoprotein Sm D3 antibody
      • SmD3 antibody
      • SMD3_HUMAN antibody
      • snRNP core protein D3 antibody
      • SNRPD3 antibody
      see all

    Images

    • All lanes : Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/5000 dilution (purified)

      Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
      Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 14 kDa



      Blocking and diluting buffer: 5% NFDM/TBST. 

    • Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/1000 dilution (purified) + NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-SNRPD3/Sm-D3 antibody [EPR7676] (ab128857) at 1/1000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 14 kDa

    References

    ab128857 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting Abcam.
    The peptide for ab128857 maps between amino acids ********of human SNRPD3 (UniProt: P62318).
    If you have any questions, please let me know

    Read More

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