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Rabbit polyclonal to SNRPG
Predicted to work with:
Mouse, Rat, Horse, Chicken, Cow, Pig, Chimpanzee, Zebrafish, Macaque monkey, Orangutan
Synthetic peptide corresponding to Human SNRPG aa 1 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P62308
This antibody gave a positive signal in HEK293 whole cell lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 8 kDa (predicted molecular weight: 8 kDa).
Appears to function in the U7 snRNP complex that is involved in histone 3'-end processing. Associated with snRNP U1, U2, U4/U6 and U5.
Belongs to the snRNP Sm proteins family.
Information by UniProt
Sm protein G antibody
Western blot - Anti-SNRPG antibody (ab111194)
Anti-SNRPG antibody (ab111194) at 1 µg/ml (Milk blocking 3%) + HEK293 (Human) Whole Cell Lysate (
) at 25 µg
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
36 kDa (possible non-specific binding), 80 kDa (possible non-specific binding)
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab111194 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
has not yet been referenced specifically in any publications.
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