Overview

  • Product name

    Anti-SNRPN antibody - N-terminal
    See all SNRPN primary antibodies
  • Description

    Rabbit polyclonal to SNRPN - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Cynomolgus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SNRPN aa 31-48 (N terminal).
    Sequence:

    FKAFDKHMNLILCDCDEF


    Database link: P63162

  • Positive control

    • WB: Rat kidney Tissue Lysate; U87, HeLa, HMY, NEUR cell lysates. IHC-P: Human Intestinal Cancer Tissue ICC/IF: HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    No cross reactivity with other proteins.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 25 kDa.
ICC Use a concentration of 0.5 - 1 µg/ml.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-SNRPN antibody - N-terminal (ab191439) at 0.5 µg/ml

    Lane 1 : Rat kidney Tissue Lysate
    Lanes 2-3 : U87 Cell Lysate
    Lane 4 : HeLa Cell Lysate
    Lane 5 : HMY Cell Lysate
    Lane 6 : NEUR Cell Lysate

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue labeling SNRPN with ab191439 at 1 μg/ml.

  • Immunocytochemical analysis of HeLa cells labeling SNRPN with ab191439 at 1 μg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human intestinal cancer tissue labeling SNRPN with ab191439 at 1 μg/mL. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling SNRPN with ab191439 at 1 μg/mL. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

References

ab191439 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
BRAIN
Blocking step
Milk as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 06 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up