This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat; HepG2; HEK293; MCF7.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 59 kDa).
1/200. Use with methanol-fixed cells.
May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.
Endosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn.
The predicted molecular weight of SNX1 is 59 kDa (SwissProt), however we expect to observe a banding pattern around 75 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Immunocytochemistry/ Immunofluorescence - Anti-SNX1 antibody (ab104366)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab104366 (1/200) staining SNX1 in HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.