Key features and details
- Rabbit polyclonal to SNX1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SNX1 antibody
See all SNX1 primary antibodies
DescriptionRabbit polyclonal to SNX1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Pig, Monkey, Orangutan
Synthetic peptide corresponding to Human SNX1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab104366 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 59 kDa).|
FunctionMay be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain.
Cellular localizationEndosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn.
- Information by UniProt
- HsT17379 antibody
- MGC8664 antibody
- SNX 1 antibody
All lanes : Anti-SNX1 antibody (ab104366) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 59 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?
The predicted molecular weight of SNX1 is 59 kDa (SwissProt), however we expect to observe a banding pattern around 75 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ab104366 has not yet been referenced specifically in any publications.