Anti-SNX1 antibody (ab995)
- Datasheet
- References (7)
- Protocols
Overview
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Product name
Anti-SNX1 antibody
See all SNX1 primary antibodies -
Description
Rabbit polyclonal to SNX1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, ICC, WBmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis -
Immunogen
Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Sorting Nexin 1 encoded within exon 15 (LocusLink ID 6642).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
ICC/IF | 1/100. | |
ICC | 1/100 - 1/500. | |
WB | 1/500 - 1/5000. |
Target
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Function
May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). -
Sequence similarities
Belongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain. -
Cellular localization
Endosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn. - Information by UniProt
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Database links
- Entrez Gene: 427480 Chicken
- Entrez Gene: 512029 Cow
- Entrez Gene: 478336 Dog
- Entrez Gene: 6642 Human
- Entrez Gene: 56440 Mouse
- Entrez Gene: 100174193 Orangutan
- Entrez Gene: 84471 Rat
- Omim: 601272 Human
see all -
Alternative names
- HsT17379 antibody
- MGC8664 antibody
- SNX 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/1000 (1µg/ml). Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/400 (2.5µg/ml). A Red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-SNX1 antibody (ab995)This image is courtesy of an anonymous Abreview
ab995 staining SNX1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Duclos CM et al. Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell Death Discov 3:16100 (2017). Human . Read more (PubMed: 28179995) »
- Yamauchi M et al. Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus. PLoS One 12:e0173017 (2017). IHC . Read more (PubMed: 28278185) »