Anti-SNX1 antibody (ab995)

Reviews (2)Q&A (3)References (7)

Overview

  • Product name
    Anti-SNX1 antibody
    See all SNX1 primary antibodies
  • Description
    Rabbit polyclonal to SNX1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Human, Zebrafish
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH - which represented a portion of human Sorting Nexin 1 encoded within exon 15 (LocusLink ID 6642).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
ICC 1/100 - 1/500.
WB 1/500 - 1/5000.

Target

  • Function
    May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
  • Sequence similarities
    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
  • Cellular localization
    Endosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • HsT17379 antibody
    • MGC8664 antibody
    • SNX 1 antibody
    • SNX 1a antibody
    • Snx1 antibody
    • SNX1_HUMAN antibody
    • SNX1A antibody
    • Sorting nexin 1 antibody
    • Sorting nexin 1A antibody
    • Sorting nexin-1 antibody
    • Vps5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/400 (2.5µg/ml). A Red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
  • Immunocytochemistry/ Immunofluorescence - Anti-SNX1 antibody (ab995)
    Immunocytochemistry/ Immunofluorescence - Anti-SNX1 antibody (ab995)This image is courtesy of an anonymous Abreview

    ab995 staining SNX1 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500)  was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Duclos CM  et al. Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell Death Discov 3:16100 (2017). Human . Read more (PubMed: 28179995) »
  • Yamauchi M  et al. Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus. PLoS One 12:e0173017 (2017). IHC . Read more (PubMed: 28278185) »
See all 7 Publications for this product

Publishing research using ab995? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-SNX1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (day 1 embryos)
Loading amount
30 µg
Specification
day 1 embryos
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Jan 21 2013

Question

How were these antibodies tested?

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Answer

Thank you for your call today and for your questions about these SNX1 antibodies.
I've heard back from the lab about these antibodies, and unfortunately there are no images available to send, but I did gather some information about the testing.
Ab995 was tested in HeLa cells and the band in Western blot was seen at approximately 70kDa. Ab76072 was tested in immunostaining on mouse cortical cell culture (1 day old) and the WB was done using rat DRG lysate.
Based on this, I think that ab76072 would be much more suitable for your assay. I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-SNX1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Dec 17 2007

Question

I am interested in knowing the peptide sequence of SNX1 against which the antibody was raised. Thank you very much.

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Answer

Development of the antigen for antibody production is a confidential/proprietary process which we do not have authority (or knowledge) to detail at this time. Our apologies.

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Question

I am planning to purchase your antibody product,SNX1(ab995) forimmunocytochemistry and western blot studies. I've looked through the paper which you said used your antibody was used for. However,Dr.Kurten et al said they raised the antibody at Bethyl Labs. Is your antibody the exact same thing as Dr.Kurten used in his study(means raised at Bethyl Lab), or you started from making the same oligo-peptide according to thier paper?

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Answer

We have recently updated our datasheets but an error in the code meant that some of our references were listed as specific rather than general. Although we are trying to clear this up it has been difficult. We thank you for bringing this one to our attention and I have adjusted the datasheet so that it now reads general reference. We are not aware of any references that cite this antibody but we always guarantee tested applications on the datasheet. Sorry for any inconvenience that this may have caused.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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