Overview

  • Product name

    Anti-SNX1 antibody [EPR7178]
    See all SNX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7178] to SNX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SNX1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • HeLa whole cell lysate (ab150035), HepG2 whole cell lysate (ab7900), 293T cell lysate, Human breast carcinoma tissue. IF/ICC: HeLa cell line.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.04 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7178
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 74 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
    • Sequence similarities

      Belongs to the sorting nexin family.
      Contains 1 BAR domain.
      Contains 1 PX (phox homology) domain.
    • Cellular localization

      Endosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn.
    • Information by UniProt
    • Database links

    • Alternative names

      • HsT17379 antibody
      • MGC8664 antibody
      • SNX 1 antibody
      • SNX 1a antibody
      • Snx1 antibody
      • SNX1_HUMAN antibody
      • SNX1A antibody
      • Sorting nexin 1 antibody
      • Sorting nexin 1A antibody
      • Sorting nexin-1 antibody
      • Vps5 antibody
      see all

    Images

    • All lanes : Anti-SNX1 antibody [EPR7178] (ab134126) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Predicted band size: 59 kDa
      Observed band size: 74 kDa
      why is the actual band size different from the predicted?

    • ab134126 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab134126 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma labelling SNX1 with ab134126 at 1/250.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Niu Y  et al. Ablation of SNX6 leads to defects in synaptic function of CA1 pyramidal neurons and spatial memory. Elife 6:N/A (2017). Read more (PubMed: 28134614) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - triton x-100
    Specification
    Brain
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2.5%
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 05 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Embryonic Fibroblast (MEF))
    Permeabilization
    Yes - 0.5% TX-100
    Specification
    Mouse Embryonic Fibroblast (MEF)
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde

    Samantha O'Hara

    Verified customer

    Submitted Oct 27 2015

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