Key features and details
- Rabbit polyclonal to SNX10
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SNX10 antibody
See all SNX10 primary antibodies
DescriptionRabbit polyclonal to SNX10
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
- IHC-P: Human cervival cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab238874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in several stages of intracellular trafficking (By similarity). May play a role in endosome homeostasis. Overexpression causes formation of huge vacuoles.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 PX (phox homology) domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol 3-phosphate.
Cellular localizationCytoplasm. Endosome membrane.
- Information by UniProt
- 2410004M09Rik antibody
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Paraffin-embedded human cervical cancer tissue stained for SNX10 with ab238874 at a 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238874 has not yet been referenced specifically in any publications.