Product nameSNX10 overexpression 293T lysate (whole cell)
ab94333 is a 293T cell transfected lysate in which Human SNX10 has been transiently over-expressed using a pCMV-SNX10 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detail about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: May be involved in several stages of intracellular trafficking (By similarity). May play a role in endosome homeostasis. Overexpression causes formation of huge vacuoles. Similarity: Belongs to the sorting nexin family. Contains 1 PX (phox homology) domain. Domain: The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3-phosphate.
Our Abpromise guarantee covers the use of ab94333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94333 at 15μg/lane on an SDS-PAGE gel
All lanes : Anti-SNX10 antibody (ab57099) at 1/500 dilution
Lane 1 :
SNX10 overexpression 293T lysate (whole cell) (ab94333)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94333 has not yet been referenced specifically in any publications.