Key features and details
- Rabbit polyclonal to SNX12
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SNX12 antibody
See all SNX12 primary antibodies
DescriptionRabbit polyclonal to SNX12
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant full length protein corresponding to Human SNX12 aa 2-172.
MSDTAVADTRRLNSKPQDLTDAYGPPSNFLEIDIFNPQTVGVGRARFTTY EVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKR QLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEA IDRNYVPGKSLAVSCPGWSAVA
Database link: Q9UMY4
- WB: PC-3 and A549 cell lysate. IHC-P: Human kidney tissue. ICC/IF: A549 cells. IP: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 20 kDa.|
|ICC/IF||1/200 - 1/500.|
|IP||1/200 - 1/2000.|
FunctionMay be involved in several stages of intracellular trafficking.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 PX (phox homology) domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol 3-phosphate.
- Information by UniProt
- 2610001F05Rik antibody
- AW045757 antibody
- MGC118982 antibody
All lanes : Anti-SNX12 antibody (ab236600) at 1/500 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20 kDa
Paraffin-embedded human kidney tissue stained for SNX12 using ab236600 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SNX12 (green) using ab236600 at 1/200 dilution in ICC/IF. Counter stained with DAPI (blue). The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. The secondary antibody was Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
SNX12 was immunoprecipitated from 0.5 mg A549 (human lung carcinoma cell line) whole cell lysate with ab236600.
Lane 1: Rabbit control IgG instead of ab236600 in A549 whole cell lysate.
Lane 2: ab236600 IP in A549 whole cell lysate.
Lane 3: A549 whole cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236600 has not yet been referenced specifically in any publications.