Product nameAnti-SNX12 antibody
See all SNX12 primary antibodies
DescriptionRabbit polyclonal to SNX12
Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant full length protein corresponding to Human SNX12 aa 2-172.
MSDTAVADTRRLNSKPQDLTDAYGPPSNFLEIDIFNPQTVGVGRARFTTY EVRMRTNLPIFKLKESCVRRRYSDFEWLKNELERDSKIVVPPLPGKALKR QLPFRGDEGIFEESFIEERRQGLEQFINKIAGHPLAQNERCLHMFLQEEA IDRNYVPGKSLAVSCPGWSAVA
Database link: Q9UMY4
- WB: PC-3 and A549 cell lysate. IHC-P: Human kidney tissue. ICC/IF: A549 cells. IP: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.03% Proclin
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 20 kDa.|
|ICC/IF||1/200 - 1/500.|
|IP||1/200 - 1/2000.|
FunctionMay be involved in several stages of intracellular trafficking.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 PX (phox homology) domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol 3-phosphate.
- Information by UniProt
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All lanes : Anti-SNX12 antibody (ab236600) at 1/500 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 2 : A549 (human lung carcinoma cell line) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 20 kDa
Paraffin-embedded human kidney tissue stained for SNX12 using ab236600 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SNX12 (green) using ab236600 at 1/200 dilution in ICC/IF. Counter stained with DAPI (blue). The cells were blocked in 10% normal goat serum and incubated with primary antibody at 4°C overnight. The secondary antibody was Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
SNX12 was immunoprecipitated from 0.5 mg A549 (human lung carcinoma cell line) whole cell lysate with ab236600.
Lane 1: Rabbit control IgG instead of ab236600 in A549 whole cell lysate.
Lane 2: ab236600 IP in A549 whole cell lysate.
Lane 3: A549 whole cell lysate.
ab236600 has not yet been referenced specifically in any publications.