Key features and details
- Rabbit polyclonal to SNX17
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SNX17 antibody
DescriptionRabbit polyclonal to SNX17
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Orangutan
Recombinant fragment corresponding to Human SNX17 aa 280-457.
DFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPL PSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSM VDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLESPDATRESM VKLSSKLSAVSLRGIGSPSTDASASDVH
Database link: Q15036
- WB: Mouse liver and kidney tissue lysates. IHC-P: Human breast cancer tissue. ICC/IF: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab223046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 50,53 kDa).|
FunctionCritical regulator of endosomal recycling of numerous receptors, channels, and other transmembrane proteins. Binds to NPxY sequences in the cytoplasmic tails of target cargos. Plays a role in the sorting of endocytosed LRP1 and APP, and prevents their degradation. Required for maintenance of normal cell surface levels of APP and LRP1. Recycles internalized integrins ITGB1, ITGB5 and their associated alpha subunits, preventing them from lysosomal degradation. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 PX (phox homology) domain.
Contains 1 Ras-associating domain.
DomainThe PX domain mediates specific binding to phosphatidylinositol 3-phosphate (PtdIns(P3)). Required for association with endosomes.
The PTB-like F3 module within the FERM-like domain mediates cargo recognition via their NPxY sequences, while the F1 module (Ras-associating) is responsible for interaction with membrane-bound HRAS.
Cellular localizationCytoplasm. Early endosome. Cytoplasmic vesicle membrane.
- Information by UniProt
- Similar to a putative C elegans gene encoded in cosmid F17H10 antibody
- Snx17 antibody
- SNX17_HUMAN antibody
All lanes : Anti-SNX17 antibody (ab223046) at 1/500 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 50,53 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SNX17 (green) using ab223046 at 1/100 dilution in ICC/IF. Secondary: Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human breast cancer tissue stained for SNX17 using ab223046 at 1/100 dilution in immunohistochemical analysis.
ab223046 has not yet been referenced specifically in any publications.