Key features and details
- Rabbit polyclonal to SNX18
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SNX18 antibody
See all SNX18 primary antibodies
DescriptionRabbit polyclonal to SNX18
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human SNX18 aa 449-628.
ANEFARKQVTGFKKEYQKVGQSFRGLSQAFELDQQAFSVGLNQAIAFTGD AYDAIGELFAEQPRQDLDPVMDLLALYQGHLANFPDIIHVQKGKAWPLEQ VIWSVLCRLKGATLTAVPLWVSESYSTGEEASRDVDAWVFSLECKLDCST GSFLLEYLALGNEYSFSKVQRVPLMTVLSF
Database link: Q96RF0
- WB: A549 and HeLa whole cell lysates; Mouse kidney lysate. IHC-P: Human tonsil and small intestine tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab237808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 69 kDa.|
FunctionMay be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain.
Contains 1 SH3 domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol-4,5-bisphosphate.
Cellular localizationEndomembrane system. Endosome membrane. Cell membrane. Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Partially colocalized with clathrin and dynamin at the cell membrane. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation.
- Information by UniProt
- FLJ11997 antibody
- FLJ32560 antibody
- FLJ61062 antibody
All lanes : Anti-SNX18 antibody (ab237808) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 69 kDa
Paraffin-embedded human tonsil tissue stained for SNX18 using ab237808 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for SNX18 using ab237808 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237808 has not yet been referenced specifically in any publications.