Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 69 kDa).Can be blocked with Human SNX18 peptide (ab22972).|
FunctionMay be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Stimulates the GTPase activity of DNM2. Promotes DNM2 location at the plasma membrane.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain.
Contains 1 SH3 domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol-4,5-bisphosphate.
Cellular localizationEndomembrane system. Endosome membrane. Cell membrane. Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Partially colocalized with clathrin and dynamin at the cell membrane. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation.
- Information by UniProt
- FLJ11997 antibody
- FLJ32560 antibody
- FLJ61062 antibody
Anti-SNX18 antibody (ab3941) at 1 µg/ml + A549 cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 69 kDa
Observed band size: 77,78 kDa why is the actual band size different from the predicted?
ab3941 has not yet been referenced specifically in any publications.