Overview

  • Product name

    Anti-SNX22 antibody
  • Description

    Rabbit polyclonal to SNX22
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 82-189 of Human SNX22.

  • Positive control

    • Human placenta tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function

    May be involved in several stages of intracellular trafficking (By similarity). Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).
  • Sequence similarities

    Belongs to the sorting nexin family.
    Contains 1 PX (phox homology) domain.
  • Domain

    The PX domain mediates specific binding to membranes enriched in phosphatidylinositol-3-phosphate (PtdIns(P3)).
  • Cellular localization

    Cytoplasmic vesicle membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • SNX22 antibody
    • SNX22_HUMAN antibody
    • Sorting nexin-22 antibody

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122477 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122477 at 1/500 dilution staining SNX22 in paraffin-embedded Human placenta tissue by Immunohistochemistry.

References

ab122477 has not yet been referenced specifically in any publications.

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