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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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RecombinantRabMAb

Recombinant Anti-SNX25 antibody [EPR9122] (ab140655)

  • Datasheet
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Western blot - Anti-SNX25 antibody [EPR9122] (ab140655)
  • Anti-SNX25 antibody [EPR9122] (ab140655)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9122] to SNX25
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human SNX25 protein (ab164461)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SNX25 antibody [EPR9122]
    See all SNX25 primary antibodies
  • Description

    Rabbit monoclonal [EPR9122] to SNX25
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human SNX25 (N terminal).

  • Positive control

    • Jurkat and Raji cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9122
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-SNX25 antibody [EPR9122] - BSA and Azide free (ab248933)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human SNX25 protein (ab164461)

Applications

Our Abpromise guarantee covers the use of ab140655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 63 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      May be involved in several stages of intracellular trafficking.
    • Sequence similarities

      Belongs to the sorting nexin family.
      Contains 1 PX (phox homology) domain.
      Contains 1 PXA domain.
      Contains 1 RGS domain.
    • Cellular localization

      Endosome membrane. Detected in endosome-derived secreted vesicles (exosomes) from malignant pleural effusions.
    • Target information above from: UniProt accession Q9H3E2 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 83891 Human
      • Entrez Gene: 102141 Mouse
      • Entrez Gene: 306471 Rat
      • SwissProt: Q9H3E2 Human
      • SwissProt: Q3ZT31 Mouse
      • Unigene: 369091 Human
      • Unigene: 267258 Mouse
      • Unigene: 16962 Rat
      • Alternative names

        • MSTP043 antibody
        • SBBI31 antibody
        • SNX25 antibody
        • SNX25_HUMAN antibody
        • Sorting nexin-25 antibody
        see all

      Images

      • Western blot - Anti-SNX25 antibody [EPR9122] (ab140655)
        Western blot - Anti-SNX25 antibody [EPR9122] (ab140655)
        All lanes : Anti-SNX25 antibody [EPR9122] (ab140655) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 63 kDa
        Observed band size: 98 kDa
        why is the actual band size different from the predicted?

      • Anti-SNX25 antibody [EPR9122] (ab140655)
        Anti-SNX25 antibody [EPR9122] (ab140655)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab140655? Please let us know so that we can cite the reference in this datasheet.

      ab140655 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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