Overview

  • Product name

    Anti-SNX27 antibody [1C6] (HRP)
    See all SNX27 primary antibodies
  • Description

    Mouse monoclonal [1C6] to SNX27 (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Fusion protein within Human SNX27 (N terminal). The exact sequence is proprietary.

  • Epitope

    N-terminal amino acids 1-267.
  • Positive control

    • WB: HeLa, HEK293 and MDA-MB-231 whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab203648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

Target

  • Function

    Involved in endocytic trafficking (By similarity). In T lymphocytes, participates in endocytic recycling pathway. Recruits PSCDBP and HT4R to early endosomes.
  • Tissue specificity

    Expressed in cells of hematopoietic origin (at protein level).
  • Sequence similarities

    Belongs to the sorting nexin family.
    Contains 1 PDZ (DHR) domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 Ras-associating domain.
  • Domain

    The PDZ domain mediates the interaction with DGKZ, PSCDBP and HT4R and is responsible for vesicular localization.
  • Cellular localization

    Cytoplasm > cytosol. Early endosome. In T-lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0488 antibody
    • Methamphetamine responsive transcript 1 antibody
    • MGC126871 antibody
    • MGC126873 antibody
    • MGC20471 antibody
    • MRT1 antibody
    • MY014 antibody
    • OTTHUMP00000042487 antibody
    • RP11-98D18.12 antibody
    • SNX27 antibody
    • SNX27_HUMAN antibody
    • Sorting nexin family member 27 antibody
    • Sorting nexin-27 antibody
    see all

Images

  • All lanes : Anti-SNX27 antibody [1C6] (HRP) (ab203648) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 61 kDa
    Observed band size: 61 kDa


    Exposure time: 1 minute


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab203648 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab203648 has not yet been referenced specifically in any publications.

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