Key features and details
- Rabbit polyclonal to SNX27
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SNX27 antibody
See all SNX27 primary antibodies
DescriptionRabbit polyclonal to SNX27
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- WB: HepG2, Jurkat and A549 whole cell lysates; Mouse brain lysate; Rat heart and liver lysates. IHC-P: Human small intestine tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 62,53 kDa (predicted molecular weight: 28,53,60,62 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionInvolved in endocytic trafficking (By similarity). In T lymphocytes, participates in endocytic recycling pathway. Recruits PSCDBP and HT4R to early endosomes.
Tissue specificityExpressed in cells of hematopoietic origin (at protein level).
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 PDZ (DHR) domain.
Contains 1 PX (phox homology) domain.
Contains 1 Ras-associating domain.
DomainThe PDZ domain mediates the interaction with DGKZ, PSCDBP and HT4R and is responsible for vesicular localization.
Cellular localizationCytoplasm > cytosol. Early endosome. In T-lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products.
- Information by UniProt
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Paraffin-embedded human small intestine tissue stained for SNX27 using ab223121 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-SNX27 antibody (ab223121) at 1/500 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat liver lysate
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 28,53,60,62 kDa
Observed band size: 53,62 kDa why is the actual band size different from the predicted?
ab223121 has not yet been referenced specifically in any publications.