Key features and details
- Rabbit polyclonal to SNX30
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SNX30 antibody
DescriptionRabbit polyclonal to SNX30
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
- Human duodenum tissue and RT4, U 251 MG, Human Liver and Tonsil lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionMay be involved in several stages of intracellular trafficking.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 PX (phox homology) domain.
- Information by UniProt
- snx30 antibody
- SNX30_HUMAN antibody
- Sorting nexin-30 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus, nucleoli and cytoplasm. Recommended concentration of ab121600 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121600 at 1/125 dilution staining SNX30 in Paraffin-embedded Human Duodenum tissue by Immunohistochemistry.
Note: strong cytoplasmic positivity in glandular cells.
All lanes : Anti-SNX30 antibody (ab121600) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U 251 MG cell lysate
Lane 3 : Human Plasma
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
ab121600 has been referenced in 1 publication.
- Simonetti B et al. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol 216:3695-3712 (2017). PubMed: 28935633