RecombinantRabMAb

Recombinant Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

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Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
  • Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
  • Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
  • Flow Cytometry - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
  • Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16954] to SNX4 - C-terminal
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SNX4 antibody [EPR16954] - C-terminal
    See all SNX4 primary antibodies

  • Description

    Rabbit monoclonal [EPR16954] to SNX4 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, A431, Jurkat, HeLa, C6, RAW 264.7, Mouse brain and PC12 cell lysates. HeLa and MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16954

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IP
Rat
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP
1/40.
Flow Cyt
1/60.
Notes
WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP
1/40.
Flow Cyt
1/60.

Target

Images

  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    All lanes : Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    All lanes : Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/1000 dilution

    Lane 1 : C6 whole cell lysate
    Lane 2 : RAW 264.7 whole cell lysate
    Lane 3 : PC12 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/10000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

    Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

    Lane 1 (Input): 293 whole cell lysate 10µg.

    Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.

    Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.


    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

    Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

    Lane 1 (Input): PC12 whole cell lysate 10µg.

    Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.

    Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.


    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow Cytometry - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Flow Cytometry - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

    Flow cytometry analysis of HeLa cells labelling SNX4 (red) with purified ab198504 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

  • Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

References (1)

Publishing research using ab198504? Please let us know so that we can cite the reference in this datasheet.

ab198504 has been referenced in 1 publication.

  • Simonetti B  et al. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol 216:3695-3712 (2017). PubMed: 28935633

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