Recombinant
RabMAb

Recombinant Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

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Overview

  • Product name

    Anti-SNX4 antibody [EPR16954] - C-terminal
    See all SNX4 primary antibodies

  • Description

    Rabbit monoclonal [EPR16954] to SNX4 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SNX4 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: O95219

  • Positive control

    • 293, A431, Jurkat, HeLa, C6, RAW 264.7, Mouse brain and PC12 cell lysates. HeLa and MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16954

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF 1/100.
IP 1/40.
Flow Cyt 1/60.

Target

Images

  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    All lanes : Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    All lanes : Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/1000 dilution

    Lane 1 : C6 whole cell lysate
    Lane 2 : RAW 264.7 whole cell lysate
    Lane 3 : PC12 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Western blot - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504) at 1/10000 dilution + Mouse brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 52 kDa
    Observed band size: 52 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

    Western blot analysis of immunoprecipitation pellet from 1 mg 293 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

    Lane 1 (Input): 293 whole cell lysate 10µg.

    Lane 2 (+) : 293 whole cell lysate using ab198504 at 1/1000 dilution.

    Lane 3 (-) : 293 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.


    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Immunoprecipitation - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

    Western blot analysis of immunoprecipitation pellet from 1 mg PC12 whole cell lysate immunoprecipitated using ab198504 at 1/40 dilution.

    Lane 1 (Input): PC12 whole cell lysate 10µg.

    Lane 2 (+) : PC12 whole cell lysate using ab198504 at 1/1000 dilution.

    Lane 3 (-) : PC12 whole cell lysate using Rabbit monoclonal IgG (ab172730) instead of ab198504.


    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

  • Flow Cytometry - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)
    Flow Cytometry - Anti-SNX4 antibody [EPR16954] - C-terminal (ab198504)

    Flow cytometry analysis of HeLa cells labelling SNX4 (red) with purified ab198504 at dilution of 1/60. The secondary antibody used was Alexa Fluor® 488 goat-anti-rabbit IgG (1/2000). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.

References

This product has been referenced in:

  • Simonetti B  et al. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol 216:3695-3712 (2017). Read more (PubMed: 28935633) »
See 1 Publication for this product

Publishing research using ab198504? Please let us know so that we can cite the reference in this datasheet.

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