Key features and details
- Rabbit polyclonal to SNX5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SNX5 antibody
See all SNX5 primary antibodies
DescriptionRabbit polyclonal to SNX5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human SNX5 aa 1-50. The exact sequence is proprietary.
Database link: Q9Y5X3
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241295 was affinity purified using an epitope specific to SNX5 immobilized on solid support.
Our Abpromise guarantee covers the use of ab241295 in the following tested applications.
|WB||1/500 - 1/2500. Predicted molecular weight: 47 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.
Sequence similaritiesBelongs to the sorting nexin family.
Contains 1 BAR domain.
Contains 1 PX (phox homology) domain.
DomainThe PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.
Cellular localizationEarly endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation.
- Information by UniProt
- 0910001N05Rik antibody
- 1810032P22Rik antibody
- AU019504 antibody
All lanes : Anti-SNX5 antibody (ab241295) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 30 seconds
Lysates prepared in NETN buffer.
SNX5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241295 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241295 at 1 µg/ml.
Lane 1: ab241295 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-295T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Lysates prepared in NETN buffer.
ab241295 has not yet been referenced specifically in any publications.