Overview

  • Product name

    Anti-SNX5 antibody [EPR14358]
    See all SNX5 primary antibodies
  • Description

    Rabbit monoclonal [EPR14358] to SNX5
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SNX5 aa 1-150. The exact sequence is proprietary.
    Database link: Q9Y5X3

  • Positive control

    • Human kidney tissue; Jurkat cells; Jurkat, HepG2 and 293T cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14358
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/60.

Target

  • Function

    May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.
  • Sequence similarities

    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
  • Domain

    The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.
  • Cellular localization

    Early endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation.
  • Information by UniProt
  • Database links

  • Alternative names

    • 0910001N05Rik antibody
    • 1810032P22Rik antibody
    • AU019504 antibody
    • D2Ertd52e antibody
    • FLJ10931 antibody
    • OTTHUMP00000030340 antibody
    • OTTHUMP00000062927 antibody
    • OTTMUSP00000003367 antibody
    • OTTMUSP00000003368 antibody
    • RP11-504H3.2 antibody
    • RP23-35E16.2 antibody
    • snoRNA MBI-43 antibody
    • SNX 5 antibody
    • SNX5 antibody
    • SNX5_HUMAN antibody
    • Sorting nexin 5 antibody
    • Sorting nexin-5 antibody
    see all

Images

  • All lanes : Anti-SNX5 antibody [EPR14358] (ab180520) at 1/10000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SNX5 with ab180520 at 1/50 dilution. Anti-rabbit HRP secondary antbody was used and cells counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunofluoresent analysis of Jurkat cells (acetone-fixed) labeling SNX5 with ab180520 at 1/100 dilution. Goat anti rabbit IgG (Dylight 488) secondary antibody was used at 1/250 dilution and cells were counterstained with DAPI.

References

This product has been referenced in:

  • Tanigawa K  et al. SNX9 determines the surface levels of integrin ß1 in vascular endothelial cells: Implication in poor prognosis of human colorectal cancers overexpressing SNX9. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30784076) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180520.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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