Anti-SNX8 antibody [OTI3C4] (ab119388)
Key features and details
- Mouse monoclonal [OTI3C4] to SNX8
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG2a
Overview
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Product name
Anti-SNX8 antibody [OTI3C4] -
Description
Mouse monoclonal [OTI3C4] to SNX8 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human SNX8 aa 1 to the C-terminus. NP_037453. Produced in HEK-293T cells.
Database link: Q9Y5X2 -
Positive control
- WB: HeLa, A549, COS-7, Jurkat and MCF7 extracts. HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA. IHC-P: Human breast adenocarcinoma, kidney, liver, bladder carcinoma and tonsil tissue. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SNX8 cDNA. Flow Cyt: Jurkat cells.
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General notes
Clone OTI3C4 (formerly 3C4).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3C4 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Flow Cyt |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ICC/IF |
1/100.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 53 kDa. |
IHC-P
1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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ICC/IF
1/100. |
Target
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Function
May be involved in several stages of intracellular trafficking. May play a role in intracellular protein transport from early endosomes to the trans-Golgi network. -
Sequence similarities
Belongs to the sorting nexin family.
Contains 1 PX (phox homology) domain. -
Cellular localization
Early endosome membrane. Colocalizes with retromer components. - Information by UniProt
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Database links
- Entrez Gene: 29886 Human
- SwissProt: Q9Y5X2 Human
- Unigene: 584900 Human
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Alternative names
- DKFZp761E1721 antibody
- Highly similar to MVP1 PROTEIN Saccharomyces cerevisiae antibody
- Mvp1 antibody
see all
Images
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All lanes : Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts
Lane 2 : A549 (Human lung carcinoma cell line) extracts
Lane 3 : COS-7 (African green monkey kidney fibroblast-like cell line) extracts
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) extracts
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) extracts
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
Paraffin-embedded human breast adenocarcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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pCMV6-ENTRY SNX8 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for SNX8 using ab119388 at a 1/100 dilution in ICC/IF.
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Flow Cytometry analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells, labeling SNX8 with ab119388 at a 1/100 dilution (red) compared to a non-specific negative control antibody (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
Paraffin-embedded human kidney tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
Paraffin-embedded human liver tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
Paraffin-embedded human bladder carcinoma tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX8 antibody [OTI3C4] (ab119388)
Paraffin-embedded human tonsil tissue stained for SNX8 with ab119388 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
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All lanes : Anti-SNX8 antibody [OTI3C4] (ab119388) at 1/200 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY SNX8 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 53 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab119388 has not yet been referenced specifically in any publications.