Anti-SOAT 1/ACAT1 antibody (ab39327)
Key features and details
- Rabbit polyclonal to SOAT 1/ACAT1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SOAT 1/ACAT1 antibody
See all SOAT 1/ACAT1 primary antibodies -
Description
Rabbit polyclonal to SOAT 1/ACAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. -
Sequence similarities
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6646 Human
- Entrez Gene: 81782 Rat
- Omim: 102642 Human
- SwissProt: P35610 Human
- SwissProt: O70536 Rat
- Unigene: 496383 Human
- Unigene: 59 Rat
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Alternative names
- ACACT antibody
- ACAT antibody
- ACAT-1 antibody
see all
Images
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All lanes : Anti-SOAT 1/ACAT1 antibody (ab39327)
Lane 1 : Rat Liver (30µg)
Lane 2 : HepG2 cell lysate (30µg)
Predicted band size: 47 kDa
Observed band size: 50,65 kDa why is the actual band size different from the predicted?
The 65 kDa band detected is the native form of ACAT1. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human adreanal tissue using ab39327 at 1/200 after heat induced antigen reteval in pH 6.0 citrate buffer. DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab39327 has been referenced in 15 publications.
- Eckhardt C et al. High expression of Sterol-O-Acyl transferase 1 (SOAT1), an enzyme involved in cholesterol metabolism, is associated with earlier biochemical recurrence in high risk prostate cancer. Prostate Cancer Prostatic Dis 25:484-490 (2022). PubMed: 34326474
- Zhong N et al. piRNA-6426 increases DNMT3B-mediated SOAT1 methylation and improves heart failure. Aging (Albany NY) 14:2678-2694 (2022). PubMed: 35354120
- Ayyagari V et al. Assessment of the diagnostic and prognostic relevance of ACAT1 and CE levels in plasma, peritoneal fluid and tumor tissue of epithelial ovarian cancer patients - a pilot study. BMC Cancer 22:387 (2022). PubMed: 35399074
- Löhr M et al. SOAT1: A Suitable Target for Therapy in High-Grade Astrocytic Glioma? Int J Mol Sci 23:N/A (2022). PubMed: 35409086
- Zhu T et al. SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis. Front Pharmacol 12:769647 (2021). PubMed: 34790132