Anti-SOAT 1/ACAT1 antibody (ab39327)

Thank you for contacting us. These two antibodies should work equally well in IHC for Human. ab53008 has not successfully been tested for other species. They do have differenct immunogenic peptides but that doesn't mean one should work better than the other. The WB images both show a band around 50 kD. The additional band you see in ab39327 is detecting the protein in the native form. They would both be guaranteed to work in human for the species lsited, respectively.

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Thank you for your reply.

Our ab39327 is actually capable of detecting both the native a processed form of the protein. I've attached the WB image and here is the caption:

Human ACAT-1 has 550 amino acids with an estimated MW of 65 kDa (1). Abcam's affinity purified antibody recognizes a 50 kDa band in most cultured cell lines as reported in the literature (2,3,4). In addition, the antibody reacts with a native form of ACAT-1 at about 65 kDa in liver tissues.

1) Chang, C.C.Y., Huh, H.Y., Cadigan, K.M., et al. J. Biol. Chem. 268(28), 20747-20755 (1993).
2) Lee, R.G., Willingham, M.C., Davis, M.A., et al. J. Lipid Res. 41, 1991 - 2001 (2000).
3) Chang, C.C.Y., Lee, C.-Y.G., Chang, E.T., et al. J. Biol. Chem. 273(52), 35132-35141 (1998).
4) Chang, C.C.Y., Chen, J., Thomas, M.A., et al. J. Biol. Chem. 270(49), 29532-29540 (1995).

I will have the WB image added to the datasheet.

I hope this information helps. Please contact us with any other questions.