Anti-SOAT 1/ACAT1 antibody (ab39327)

Submit a review Q&A (2)References (6)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOAT 1/ACAT1 antibody (ab39327)
  • Western blot - Anti-SOAT 1/ACAT1 antibody (ab39327)

Key features and details

  • Rabbit polyclonal to SOAT 1/ACAT1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

Overview

  • Product name

    Anti-SOAT 1/ACAT1 antibody
    See all SOAT 1/ACAT1 primary antibodies

  • Description

    Rabbit polyclonal to SOAT 1/ACAT1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Cynomolgus monkey

  • Immunogen

    Synthetic peptide corresponding to Human SOAT 1/ACAT1 aa 6-23.
    Sequence:

    KMSRLNRLSKSRENPEED

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

     This product was previously labelled as SOAT 1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab39327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.

  • Sequence similarities

    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ACACT antibody
    • ACAT antibody
    • ACAT-1 antibody
    • ACAT1 antibody
    • Acyl coenzyme A cholesterol acyltransferase 1 antibody
    • Acyl-coenzyme A:cholesterol acyltransferase 1 antibody
    • Cholesterol acyltransferase 1 antibody
    • SOAT antibody
    • SOAT1 antibody
    • SOAT1_HUMAN antibody
    • STAT antibody
    • Sterol O acyltransferase 1 antibody
    • Sterol O-acyltransferase 1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOAT 1/ACAT1 antibody (ab39327)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOAT 1/ACAT1 antibody (ab39327)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human adreanal tissue using ab39327 at 1/200 after heat induced antigen reteval in pH 6.0 citrate buffer. DAB staining.

  • Western blot - Anti-SOAT 1/ACAT1 antibody (ab39327)
    Western blot - Anti-SOAT 1/ACAT1 antibody (ab39327)
    All lanes : Anti-SOAT 1/ACAT1 antibody (ab39327)

    Lane 1 : Rat Liver (30µg)
    Lane 2 : HepG2 cell lysate (30µg)

    Predicted band size: 47 kDa
    Observed band size: 50,65 kDa
    why is the actual band size different from the predicted?



    The 65 kDa band detected is the native form of ACAT1.

References (6)

Publishing research using ab39327? Please let us know so that we can cite the reference in this datasheet.

ab39327 has been referenced in 6 publications.

  • Chortis V  et al. Mitotane treatment in patients with metastatic testicular Leydig cell tumor associated with severe androgen excess. Eur J Endocrinol 178:K21-K27 (2018). PubMed: 29330226
  • Sundberg JP  et al. Sebaceous gland abnormalities in fatty acyl CoA reductase 2 (Far2) null mice result in primary cicatricial alopecia. PLoS One 13:e0205775 (2018). PubMed: 30372477
  • Pera M  et al. Increased localization of APP-C99 in mitochondria-associated ER membranes causes mitochondrial dysfunction in Alzheimer disease. EMBO J 36:3356-3371 (2017). PubMed: 29018038
  • Potter CS  et al. Dysregulated expression of sterol O-acyltransferase 1 (Soat1) in the hair shaft of Hoxc13 null mice. Exp Mol Pathol 99:441-4 (2015). PubMed: 26321246
  • Sundberg JP  et al. Immune status, strain background, and anatomic site of inoculation affect mouse papillomavirus (MmuPV1) induction of exophytic papillomas or endophytic trichoblastomas. PLoS One 9:e113582 (2014). PubMed: 25474466
  • Area-Gomez E  et al. Upregulated function of mitochondria-associated ER membranes in Alzheimer disease. EMBO J 31:4106-23 (2012). PubMed: 22892566

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question


I am sorry to trouble youagain，but I am still wondering the difference of the two products of yours，Anti-SOAT 1 antibody [EP1376Y] (ab53008) and Anti-SOAT 1 antibody (ab39327).Since they detect different bands in Immunoblot analysis，and they have different manufacturing process(method :Synthetic peptide: KMSRLNRLSKSRENPEED, corresponding to amino acids 6/23 of Human ACAT1 and A synthetic peptide corresponding to residues on the C-terminus of human ACAT)，i wonder what is the difference between the two when they are used in IHC.

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Answer

Thank you for contacting us. These two antibodies should work equally well in IHC for Human. ab53008 has not successfully been tested for other species. They do have differenct immunogenic peptides but that doesn't mean one should work better than the other. The WB images both show a band around 50 kD. The additional band you see in ab39327 is detecting the protein in the native form. They would both be guaranteed to work in human for the species lsited, respectively.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

There is another product of your company Anti-SOAT 1 antibody (ab39327) that detects a band at 47 kDa（Predicted molecular weight: 47 kDa）.

So does it detect a fragment of ACAT-1 or detect the socalled processed form of the protein ？

Since i didn't find any article that talk about ACAT fragment or processed form，i wonder if i can be informed of more detailed information about your product ACAT-1 antibody(aka SOAT 1).

Thanks for your patience,your reply will be so appreciated.

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Answer

Thank you for your reply.

Our ab39327 is actually capable of detecting both the native a processed form of the protein. I've attached the WB image and here is the caption:

Human ACAT-1 has 550 amino acids with an estimated MW of 65 kDa (1). Abcam's affinity purified antibody recognizes a 50 kDa band in most cultured cell lines as reported in the literature (2,3,4). In addition, the antibody reacts with a native form of ACAT-1 at about 65 kDa in liver tissues.

1) Chang, C.C.Y., Huh, H.Y., Cadigan, K.M., et al. J. Biol. Chem. 268(28), 20747-20755 (1993).
2) Lee, R.G., Willingham, M.C., Davis, M.A., et al. J. Lipid Res. 41, 1991 - 2001 (2000).
3) Chang, C.C.Y., Lee, C.-Y.G., Chang, E.T., et al. J. Biol. Chem. 273(52), 35132-35141 (1998).
4) Chang, C.C.Y., Chen, J., Thomas, M.A., et al. J. Biol. Chem. 270(49), 29532-29540 (1995).

I will have the WB image added to the datasheet.

I hope this information helps. Please contact us with any other questions.

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