Anti-SOAT 1/ACAT1 antibody (ab39327)
Key features and details
- Rabbit polyclonal to SOAT 1/ACAT1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-SOAT 1/ACAT1 antibody
See all SOAT 1/ACAT1 primary antibodies -
Description
Rabbit polyclonal to SOAT 1/ACAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Cynomolgus monkey -
Immunogen
Synthetic peptide corresponding to Human SOAT 1/ACAT1 aa 6-23.
Sequence:KMSRLNRLSKSRENPEED
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General notes
This product was previously labelled as SOAT 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab39327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. | |
IHC-P | Use at an assay dependent concentration. |
Target
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Function
Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. -
Sequence similarities
Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6646 Human
- Entrez Gene: 20652 Mouse
- Entrez Gene: 81782 Rat
- Omim: 102642 Human
- SwissProt: P35610 Human
- SwissProt: Q61263 Mouse
- SwissProt: O70536 Rat
- Unigene: 496383 Human
see all -
Alternative names
- ACACT antibody
- ACAT antibody
- ACAT-1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOAT 1/ACAT1 antibody (ab39327)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human adreanal tissue using ab39327 at 1/200 after heat induced antigen reteval in pH 6.0 citrate buffer. DAB staining.
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All lanes : Anti-SOAT 1/ACAT1 antibody (ab39327)
Lane 1 : Rat Liver (30µg)
Lane 2 : HepG2 cell lysate (30µg)
Predicted band size: 47 kDa
Observed band size: 50,65 kDa why is the actual band size different from the predicted?
The 65 kDa band detected is the native form of ACAT1.
Protocols
Datasheets and documents
References (6)
ab39327 has been referenced in 6 publications.
- Chortis V et al. Mitotane treatment in patients with metastatic testicular Leydig cell tumor associated with severe androgen excess. Eur J Endocrinol 178:K21-K27 (2018). PubMed: 29330226
- Sundberg JP et al. Sebaceous gland abnormalities in fatty acyl CoA reductase 2 (Far2) null mice result in primary cicatricial alopecia. PLoS One 13:e0205775 (2018). PubMed: 30372477
- Pera M et al. Increased localization of APP-C99 in mitochondria-associated ER membranes causes mitochondrial dysfunction in Alzheimer disease. EMBO J 36:3356-3371 (2017). PubMed: 29018038
- Potter CS et al. Dysregulated expression of sterol O-acyltransferase 1 (Soat1) in the hair shaft of Hoxc13 null mice. Exp Mol Pathol 99:441-4 (2015). PubMed: 26321246
- Sundberg JP et al. Immune status, strain background, and anatomic site of inoculation affect mouse papillomavirus (MmuPV1) induction of exophytic papillomas or endophytic trichoblastomas. PLoS One 9:e113582 (2014). PubMed: 25474466
- Area-Gomez E et al. Upregulated function of mitochondria-associated ER membranes in Alzheimer disease. EMBO J 31:4106-23 (2012). PubMed: 22892566