Overview

  • Product name

    Anti-SOAT 1/ACAT1 antibody
    See all SOAT 1/ACAT1 primary antibodies
  • Description

    Rabbit polyclonal to SOAT 1/ACAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human SOAT 1/ACAT1 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91082)

  • Positive control

    • This antibody gave a positive signal in the following lysates: Mouse Spleen Tissue; Ramos Whole Cell; RAW264.7 Whole Cell (data not shown).
  • General notes

     This product was previously labelled as SOAT 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab72229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 65 kDa).

Target

  • Function

    Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.
  • Sequence similarities

    Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACACT antibody
    • ACAT antibody
    • ACAT-1 antibody
    • ACAT1 antibody
    • Acyl coenzyme A cholesterol acyltransferase 1 antibody
    • Acyl-coenzyme A:cholesterol acyltransferase 1 antibody
    • Cholesterol acyltransferase 1 antibody
    • SOAT antibody
    • SOAT1 antibody
    • SOAT1_HUMAN antibody
    • STAT antibody
    • Sterol O acyltransferase 1 antibody
    • Sterol O-acyltransferase 1 antibody
    see all

Images

  • All lanes : Anti-SOAT 1/ACAT1 antibody (ab72229) at 1 µg/ml

    Lane 1 : Spleen (Mouse) Tissue Lysate
    Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 65 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    The 55 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOAT1/ACAT1.

References

ab72229 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab72229.
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