Overview

  • Product name

  • Description

    Rabbit polyclonal to SOAT 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human SOAT 2 aa 1-120.
    Database link: O75908

  • Positive control

    • WB: HEK-293T whole cell lysate. IHC-P: Human liver and small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab230220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60, 58, 50, 35 kDa).

Target

  • Relevance

    Acyl-coenzyme A: cholesterol acyltransferase 2 (SOAT2/ACAT2) catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. It is associated with lipoprotein particle secretion and thus plays an important role in the metabolism of triglyceride-rich lipoproteins like ApoB100. In humans, ACAT2 is primarily expressed in the small intestine, whereas in other mammals, the enzyme is expressed in the liver and the small intestine. Human ACAT2 has 522 amino acids with an estimated molecular weight of 60 kDa
  • Cellular localization

    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • ACACT2 antibody
    • ACAT 2 antibody
    • ACAT2 antibody
    • Acyl Co A: cholesterol acyltransferase 2 antibody
    • Acyl CoA:cholesterol acyltransferase 2 antibody
    • acyl coenzyme A cholestrol acyltransferase 2 antibody
    • Acyl coenzyme A:cholesterol acyltransferase 2 antibody
    • ARGP2 antibody
    • Cholesterol acyltransferase 2 antibody
    • Sterol O acyl transferase 2 antibody
    see all

Images

  • Anti-SOAT 2 antibody (ab230220) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary
    Goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 60, 58, 50, 35 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded human liver tissue stained for SOAT 2 using ab230220 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human small intestine tissue stained for SOAT 2 using ab230220 at 1/100 dilution in immunohistochemical analysis.

References

ab230220 has not yet been referenced specifically in any publications.

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