• Product name
  • Description
    Rabbit polyclonal to SOCS1
  • Host species
  • Specificity
    By Western blot, this antibody detects a band of approximately 24 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 1. However, in human spleen cell lysate a band at 37kDa is observed.
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 199-211 of human SOCS 1 or 200-212 of mouse SOCS 1.

  • Positive control
    • human spleen lysate



Our Abpromise guarantee covers the use of ab3691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 19435411

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa).
ELISA Use a concentration of 0.1 - 1 µg/ml.


  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • Tissue specificity
    Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • Database links
  • Alternative names
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all


  • All lanes : Anti-SOCS1 antibody (ab3691)

    Lane 1 : K562 cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : K562 cell lysate with Immunizing peptide
    Lane 4 : Mouse spleen tissue lysate with Immunizing peptide

    Predicted band size: 24 kDa


This product has been referenced in:
  • Chuang Y  et al. Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immun 22:647-657 (2016). WB ; Mouse . Read more (PubMed: 27670945) »
  • Webster KE  et al. IL-17-producing NKT cells depend exclusively on IL-7 for homeostasis and survival. Mucosal Immunol N/A:N/A (2014). Human . Read more (PubMed: 24448098) »

See all 18 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Loading amount
0.8 cells
Gel Running Conditions
Reduced Denaturing (12%)
Human Cell lysate - whole cell (primary human monocyte)
primary human monocyte
MDP 100ug/ml
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2013

Flow Cytometry
Human Cell (293T)
BD Cytofix/Cytoperm solution
Yes - BD Cytofix/Cytoperm solution
Gating Strategy
no gating

Mrs. Duo Li

Verified customer

Submitted May 02 2013


Expiration date: 16. February 2013

Value: EURO 360.-

I am very pleased to hear you would like to use ab3691 in flow cytometry and send us an image of your results.

This code will give...

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Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.


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Thank you for contacting us and for your interest in our products.

As far as we are aware, ab3691 Anti-SOCS1 antibody has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as a...

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I apologize for the delay in finding out the results of the test of the antibody, I am pleased to inform you that we have re-tested this lot against our new lot and found that its activity has decreased over time. We would therefore like to offer you a...

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Thank you for your enquiry. The HEK cells were incubated with 500 ng/mL human growth hormone for 6-12 hours. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. The stimulation conditions for the HEK293 cells seen in the Western blot image are 500 ng/ml human growth hormone. I hope this information helps, please do not hesitate to contact us if you need any more advice or informa...

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Thank you for getting back in touch with me. As mentioned in my previous email I consider SOCS1 peptide (199-211) (ab23167) suitable as a positive control standard for ab3691 as this is the very peptide sequence that the antibody was raised against....

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Thank you for your enquiry. Unfortunately we do no have a recommended positive control for SOCS1 antibody (ab3691). However, the customer that left a favourable Abreview used a human cell lysate - whole cell (human malignant melanoma cells). Furthe...

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