Overview

  • Product name
  • Description
    Rabbit polyclonal to SOCS1
  • Host species
    Rabbit
  • Specificity
    By Western blot, this antibody detects a band of approximately 24 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 1. However, in human spleen cell lysate a band at 37kDa is observed.
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    VLRDYLSSFPFQI

    , corresponding to amino acids 199-211 of human SOCS 1 or 200-212 of mouse SOCS 1.

  • Positive control
    • human spleen lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab3691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 19435411

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa).
ELISA Use a concentration of 0.1 - 1 µg/ml.

Target

  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • Tissue specificity
    Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • Database links
  • Alternative names
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all

Images

  • All lanes : Anti-SOCS1 antibody (ab3691)

    Lane 1 : K562 cell lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : K562 cell lysate with Immunizing peptide
    Lane 4 : Mouse spleen tissue lysate with Immunizing peptide

    Predicted band size: 24 kDa

References

This product has been referenced in:
  • Chuang Y  et al. Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immun 22:647-657 (2016). WB ; Mouse . Read more (PubMed: 27670945) »
  • Webster KE  et al. IL-17-producing NKT cells depend exclusively on IL-7 for homeostasis and survival. Mucosal Immunol N/A:N/A (2014). Human . Read more (PubMed: 24448098) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 15 Abreviews or Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
0.8 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (primary human monocyte)
Specification
primary human monocyte
Treatment
MDP 100ug/ml
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 10 2013

Abreviews
Application
Flow Cytometry
Sample
Human Cell (293T)
Specification
293T
Fixation
BD Cytofix/Cytoperm solution
Permeabilization
Yes - BD Cytofix/Cytoperm solution
Gating Strategy
no gating

Mrs. Duo Li

Verified customer

Submitted May 02 2013

Question
Answer

DISCOUNT CODE: 100%ABR-00000

Expiration date: 16. February 2013

Value: EURO 360.-

I am very pleased to hear you would like to use ab3691 in flow cytometry and send us an image of your results.

This code will give you EURO 360.- off a future order order before the expiration date. To redeem this offer, please submit an Abreview with the image for flow cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

https://www.abcam.com/index.html?pageconfig=resource&rid=11380

https://www.abcam.com/index.html?pageconfig=resource&rid=11448

https://www.abcam.com/index.html?pageconfig=resource&rid=11381

https://www.abcam.com/index.html?pageconfig=popular_protocols

The optimal dilutions should be determined by end user.I wouldsuggest starting anywhere from1ug-10ug/ml.

All of these antibodies are tested with human samples and in IHC-P e.g. ab62584, ab9870, ab135718, ab83493, ab65989 so these would work. Choose any one of these for best results. Please note,because you are using mouse for tissue grafts so avoiding mouse monoclonal would be a good idea else we can suggest some extra blocking steps.

The concentration of antibody for IHC-P can be tried somewhere between 1-10ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab3691 Anti-SOCS1 antibody has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our attention. Therefore, according to our datasheet the antibodies has successfully tested in ICC/IF and is covered by our Abpromise with human and mouse samples.

We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact me for any further advice or information in this regard.

Read More

Answer

I apologize for the delay in finding out the results of the test of the antibody, I am pleased to inform you that we have re-tested this lot against our new lot and found that its activity has decreased over time. We would therefore like to offer you a replacement vial of the new lot, which was just tested and gave a good band at 37kDa in human spleen lysate. I have added the image of the test on the datasheet immediately for you to see. I am very sorry it has taken unusually long to resolve this matter. I would appreciate if you could confirm if you would be interested in trying the new lot, or if you would prefer a refund on your order 250110. Thank you very much in advance,

Read More

Answer

Thank you for your enquiry. The HEK cells were incubated with 500 ng/mL human growth hormone for 6-12 hours. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. The stimulation conditions for the HEK293 cells seen in the Western blot image are 500 ng/ml human growth hormone. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for getting back in touch with me. As mentioned in my previous email I consider SOCS1 peptide (199-211) (ab23167) suitable as a positive control standard for ab3691 as this is the very peptide sequence that the antibody was raised against. With regards mouse monoclonal [SO1] to SOCS3 (ab14939) we do not have a positive control in our catalogue. However, following a brief "Google" search I can tell you I found an epitope tagged recombinant human form of SOCS3 available at the following link; http://www.axxora.com/socs-related_products-ALX-201-334/opfa.1.1.ALX-201-334.1872.4.1.html I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry. Unfortunately we do no have a recommended positive control for SOCS1 antibody (ab3691). However, the customer that left a favourable Abreview used a human cell lysate - whole cell (human malignant melanoma cells). Furthermore Ramos or RAW cells are recommended according to the literature. With respect to SOCS2 antibody (ab3692) we recommend the use of lysates of GH treated HEK 293 cells transfected with GHR. Furthermore in the following publication that has used this antibody C57BL/6 mice overexpressing a SOCS2Tg were employed for use by IHC. Scott HJ et al. Differential effects of SOCS2 on neuronal differentiation and morphology. Brain Res 1067:138-45 (2006). PubMed: 16360125 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

1-10 of 15 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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