Product nameAnti-SOCS1 antibody
See all SOCS1 primary antibodies
DescriptionRabbit polyclonal to SOCS1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to a 17 amino acid sequence near the N terminal of human SOCS1.
- Human spleen cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 24 kDa).|
FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
Tissue specificityExpressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
- Information by UniProt
- CISH 1 antibody
- CISH1 antibody
- Cytokine inducible SH2 protein 1 antibody
Lane 1 : Anti-SOCS1 antibody (ab37167) at 1 µg/ml
Lane 2 : Anti-SOCS1 antibody (ab37167) at 2 µg/ml
Lane 3 : Anti-SOCS1 antibody (ab37167) at 4 µg/ml
All lanes : Human spleen cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Raad H et al. Thyroid hydrogen peroxide production is enhanced by the Th2 cytokines, IL-4 and IL-13, through increased expression of the dual oxidase 2 and its maturation factor DUOXA2. Free Radic Biol Med : (2012). Read more (PubMed: 23010498) »
- Platten M et al. Blocking angiotensin-converting enzyme induces potent regulatory T cells and modulates TH1- and TH17-mediated autoimmunity. Proc Natl Acad Sci U S A : (2009). WB ; Mouse . Read more (PubMed: 19706421) »