Key features and details
- Rabbit polyclonal to SOCS1 - Carboxyterminal end
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SOCS1 antibody - Carboxyterminal end
See all SOCS1 primary antibodies
DescriptionRabbit polyclonal to SOCS1 - Carboxyterminal end
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic peptide corresponding to a sequence at the C-terminal of human SOCS1, identical to the related mouse and rat sequence.
- Rat lung tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml.|
FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
Tissue specificityExpressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
- Information by UniProt
- CISH 1 antibody
- CISH1 antibody
- Cytokine inducible SH2 protein 1 antibody
ab65989, at 0.5µg/ml, staining SOCS1 in paraffin-embedded rat lung tissue by immunohistochemistry. Staining of SOCS1 in the nucleus is observed. DAB chromogenic reaction.
All lanes : Anti-SOCS1 antibody - Carboxyterminal end (ab65989)
Lane 1 : HT1080 Cell Lysate
Lane 2 : COLO320 Cell Lysate
Predicted band size: 24 kDa
ab65989 has been referenced in 1 publication.
- Muratsu D et al. Zoledronic acid enhances lipopolysaccharide-stimulated proinflammatory reactions through controlled expression of SOCS1 in macrophages. PLoS One 8:e67906 (2013). WB . PubMed: 23874464