Anti-SOCS1 antibody - N-terminal (ab135718)

Rabbit polyclonal SOCS1 antibody. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Human. Immunogen corresponding to synthetic peptide.

Overview

  • Product name

    Anti-SOCS1 antibody - N-terminal
    See all SOCS1 primary antibodies
  • Description

    Rabbit polyclonal to SOCS1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 43-73 of Human SOCS1 (UniProt ID: O15524).

  • Positive control

    • Human colon carcinoma; Mouse kidney tissue lysates; 293 cells; WiDr cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab135718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/10 - 1/50.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/50 - 1/100. Predicted molecular weight: 23 kDa.

Target

  • Function

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • Tissue specificity

    Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain

    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • Database links

  • Alternative names

    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all

Images

  • Anti-SOCS1 antibody - N-terminal (ab135718) at 1/50 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size: 23 kDa

  • Flow cytometric analysis of SOCS1 expression in WiDr cells labelled with ab135718 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • Immunofluorescence analysis of SOCS1 expression in 293 cells labelled with ab135718 at 1/10 dilution. This was followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • Immunohistochemical analysis of SOCS1 expression in paraffin embedded, formalin fixed Human colon carcinoma tissue labelled with ab135718 at 1/50 dilution. This was followed by incubation with a peroxidase conjugated secondary antibody and DAB staining.

References

ab135718 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

https://www.abcam.com/index.html?pageconfig=resource&rid=11380

https://www.abcam.com/index.html?pageconfig=resource&rid=11448

https://www.abcam.com/index.html?pageconfig=resource&rid=11381

https://www.abcam.com/index.html?pageconfig=popular_protocols

The optimal dilutions should be determined by end user.I wouldsuggest starting anywhere from1ug-10ug/ml.

All of these antibodies are tested with human samples and in IHC-P e.g. ab62584, ab9870, ab135718, ab83493, ab65989 so these would work. Choose any one of these for best results. Please note,because you are using mouse for tissue grafts so avoiding mouse monoclonal would be a good idea else we can suggest some extra blocking steps.

The concentration of antibody for IHC-P can be tried somewhere between 1-10ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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