Overview

  • Product name
  • Description
    Rabbit polyclonal to SOCS2
  • Host species
    Rabbit
  • Specificity
    By Western blot, this antibody detects a band of approximately 23 kDa in lysate of GH treated HEK 293 cells transfected with GHR, which corresponds to the predicted molecular weight of SOCS 2.
  • Tested applications
    Suitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (

    PTRLKDYLEEYKFQV

    ) corresponding to amino acids 184-198 of human, rat and mouse SOCS 2.

  • Positive control
    • lysate of GH treated HEK 293 cells transfected with GHR, E17 mouse cortical neuron lysate (see Scott et al)
  • General notes


    Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.

Target

  • Function
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • Tissue specificity
    High expression in heart, placenta, lung, kidney and prostate.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • Domain
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt
  • Database links
  • Alternative names
    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Images

  • Representative western blot staining SOCS2 with ab3692 at 1/100 dilution, in Human peripheral blood mononuclear cells of a healthy control (blood sample taken before pregnancy) and a MS patient (blood samples taken before and during pregnancy).

References

This product has been referenced in:
  • Li B  et al. Development and Validation of a Three-gene Prognostic Signature for Patients with Hepatocellular Carcinoma. Sci Rep 7:5517 (2017). WB . Read more (PubMed: 28717245) »
  • Gurda GT  et al. Profiling CCK-mediated pancreatic growth: the dynamic genetic program and the role of STATs as potential regulators. Physiol Genomics 44:14-24 (2012). WB ; Mouse . Read more (PubMed: 22010007) »
See all 10 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 24 h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (primary human macrophages)
Specification
primary human macrophages
Treatment
MDP 100ug/ml
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Dr. sha zheng

Verified customer

Submitted Jun 18 2013

Answer

Thank you for passing on this enquiry from the customer. I hope you are well. All our antibodies that have been tested western blotting will detect reduced proteins, unless otherwise stated the datasheet. The customer will therefore need to reduce their samples. I hope this helps. I wish the customer good luck with the experiments!

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Answer

Thank you for getting back to me with your customer's further information. Please can you pass on the following comments to your customer: I am surprised that you have been unable to detect consistent hypothalamus staining using this antibody. It is most strange that you have observed a decline in the quality of your immunolabelling. Can you tell me how the antibody was stored. Were aliquots prepared following receipt of the antibody and stored at -20 or -80oC? Could the variation in the results that you have been obtaining be attributable to the method of preparation of the cells. Were protease inhibitors employed during the preparation? I look forward to hearing from your customer.

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Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Rat retinal protein lysate PRIMARY ANTIBODY Abcam (SOCS2; ab3692)/Rabbit/TBS 0.0% Tween 20/2.0ug/ml/overnight at 4 degree C/3X5minute washes with TBST DETECTION METHOD Supersignal West Pico chemiluminescent substrate POSITIVE AND NEGATIVE CONTROLS USED Negative control-no primary control or retinal cells untreated with CNTF Positive control- retinal cells treated with CNTF ANTIBODY STORAGE CONDITIONS -20 degree Celsius/PBS with 0.02% sodium azide (as arrived SAMPLE PREPARATION (1% Triton X, 0.5% NP-40, 1mM EDTA, 150mM NaCl, 1mM PMSF, 5mM sodium pyrophosphate, 0.2mM sodium molybdate, 0.05mM sodium fluoride and 1mM sodium orthovanadate), cell supernatant was collected at 15,000 X g for 15 minutes, snap frozen and kept at ?80?C for later usage. Protease Inhibitor added just before homogenization. Protein and sample buffer mixture heated at 95C for 5 minutes AMOUNT OF PROTEIN LOADED 50-100ug per lane ELECTROPHORESIS/GEL CONDITIONS 10% acrylamide/Bis solution (Bio-Rad) gel/ electrophoresis at 120V for 2-3 hours. TRANSFER AND BLOCKING CONDITIONS Transfer buffer (3.03g Tris, 14.4g glycine, 200ml methanol)/ transfer at 100V for 1 hours Blocked in 5% skim milk in 0.1% Tween 20 (ICN, Ohio, USA) in TBS SECONDARY ANTIBODY Pierce/Goat anti-rabbit HRP/TBST/1:5,000-1:20,000/1-3hours at RT or overnight at 4C/3X5minute TBST wash HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? - varying amount of samples loaded, different gel concentration, shorter wash step, different blocking buffers, different dilutions of primary + secondary antibodies, different secondary antibodies, different ECL

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Answer

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Quality is very important to Abcam. We take all of our complaints very seriously and will not hesitate to remove an antibody should we have concerns over the quality of the antibody. I have read your questionaire and I have a few comments. YOu mention that you are using rat retinal lysate. However, according to the Expasy database the level of SOCS2 expression in mouse retina is low; with regions of the brain showing moderate to high levels of expression dependent on the stage of development. Please can you tell me whether you have confirmed expression of the transcript in your samples. You are also using CNTF to induce your positive control cells. However, from some literature searching I found that "CNTF (Bjorbaek et al., 1999b) significantly increases SOCS3, but not CIS, SOCS1, or SOCS2, gene expression in the circumventricular organs and the hypothalamus". (Wang J, Campbell IL. J Neurosci Res. 2002 Feb 15;67(4):423-7. Cytokine signaling in the brain: putting a SOCS in it?). Whilst I understand that this is the brain and expression may be different in the retina I would recommend that any induction is assayed at the transcript level in addition to the protein level as you are doing here. I would like to recommend that you use rat hypothalamus tissue as the positive control in this instance. I would appreciate your comments on the above and look forward to hearing from you.

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Answer

Thank you for your enquiry. Unfortunately we do no have a recommended positive control for SOCS1 antibody (ab3691). However, the customer that left a favourable Abreview used a human cell lysate - whole cell (human malignant melanoma cells). Furthermore Ramos or RAW cells are recommended according to the literature. With respect to SOCS2 antibody (ab3692) we recommend the use of lysates of GH treated HEK 293 cells transfected with GHR. Furthermore in the following publication that has used this antibody C57BL/6 mice overexpressing a SOCS2Tg were employed for use by IHC. Scott HJ et al. Differential effects of SOCS2 on neuronal differentiation and morphology. Brain Res 1067:138-45 (2006). PubMed: 16360125 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

I have received feedback from the source of ab3692 stating that unfortunately they do not know if the cells were transfected with the receptor or not. We do not have the stimulation paradigm information unfortunately, I'm very sorry. From your literature search I would be inclined to say that the cells are likely to be transfected, would you be so kind to provide those references so I can read those? We do not know of a good recommended positive control. Maybe the current literature will have such information? Thank you and my apologies for not being able to help you more.

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