Product nameAnti-SOCS2 antibody
See all SOCS2 primary antibodies
DescriptionRabbit polyclonal to SOCS2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Chimpanzee
Synthetic peptide corresponding to Human SOCS2 aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: HepG2, HeLa, SW480, Raji, MOLT4 and in Mouse Thymus tissue lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab66733 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Tissue specificityHigh expression in heart, placenta, lung, kidney and prostate.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
DomainThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
- Information by UniProt
- CIS 2 antibody
- CIS-2 antibody
- CIS2 antibody
All lanes : Anti-SOCS2 antibody (ab66733) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 :
SW480 whole cell lysate (ab3957)
Lane 4 :
Raji whole cell lysate (ab30124)
Lane 5 :
Mouse thymus tissue lysate - total protein (ab29285)
Lane 6 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
ab66733 has not yet been referenced specifically in any publications.