RecombinantRabMAb

Recombinant Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

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Western blot - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
  • Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
  • Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
  • Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
  • Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2588(2)] to SOCS2
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SOCS2 antibody [EPR2588(2)]
    See all SOCS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2588(2)] to SOCS2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IP, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human SOCS2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: NCCIT and K-562 whole cell lysates; IP: K-562 whole cell lysate; Flow Cyt: HeLa cells; ICC: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/150.

Target

  • Function

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

  • Tissue specificity

    High expression in heart, placenta, lung, kidney and prostate.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.

  • Domain

    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Images

  • Western blot - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Western blot - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    All lanes : Anti-SOCS2 antibody [EPR2588(2)] (ab109245) at 1/2000 dilution (purified)

    Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
    Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Immunocytochemistry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/100 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Flow Cytometry - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SOCS2 with purified ab109245 at 1/150 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Immunoprecipitation - Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

    Purified ab109245 at 1/70 dilution (2 µg) immunoprecipitating SOCS2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 µg
    Lane 2 (+): ab109245 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109245 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 22 kDa

  • Anti-SOCS2 antibody [EPR2588(2)] (ab109245)
    Anti-SOCS2 antibody [EPR2588(2)] (ab109245)

References (0)

Publishing research using ab109245? Please let us know so that we can cite the reference in this datasheet.

ab109245 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Submit a question

Question

The antibody detects a strong band at 75 kDa, in addition to a band at the expected 22 kDa. We need to know what this 75 kDa band represents, in order to use the antibody in an antibody array.

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I have issued a free of charge replacement, ab92847.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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