Anti-SOCS3 antibody (ab16030)
- Datasheet
- References (60)
- Protocols
Overview
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Product nameAnti-SOCS3 antibody
See all SOCS3 primary antibodies -
DescriptionRabbit polyclonal to SOCS3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Dog
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SOCS3.
Read Abcam's proprietary immunogen policy (Peptide available as ab16199.) -
Positive control
- This antibody gave a positive signal in the following Human Whole Cell Lysates: Hela, Jurkat, A431 and MOLT4.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab16030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| Flow Cyt | 1/300. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24.7 kDa).Can be blocked with Human SOCS3 peptide (ab16199). | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 19855398 | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
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Tissue specificityWidely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
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PathwayProtein modification; protein ubiquitination.
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Involvement in diseaseNote=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
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Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain. -
DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. -
Post-translational
modificationsPhosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. - Information by UniProt
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Database links
- Entrez Gene: 395299 Chicken
- Entrez Gene: 282081 Cow
- Entrez Gene: 442949 Dog
- Entrez Gene: 9021 Human
- Entrez Gene: 12702 Mouse
- Entrez Gene: 89829 Rat
- Omim: 604176 Human
- SwissProt: Q90X67 Chicken
see all -
Alternative names
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
see all
Images
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Western blot - Anti-SOCS3 antibody (ab16030)The 30kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to SOCS3. -
Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody (ab16030)ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody (ab16030)Image courtesy of Human Protein Atlas
ab16030 staining SOCS3 in human bone marrow showing strong staining within the bone marrow. Paraffin embedded human bone marrow was incubated with ab16030 (1/100) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH6.
ab16030 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
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Immunohistochemistry (Frozen sections) - Anti-SOCS3 antibody (ab16030)This image is courtesy of an anonymous Abreviewab16030 staining SOCS3 in Human pancreatic tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI. -
Western blot - Anti-SOCS3 antibody (ab16030)This image is courtesy of an anonymous AbreviewAll lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml
Lane 1 : Untreated Human peripheral blood mononuclear cell lysate
Lane 2 : Human peripheral blood mononuclear cell lysate (treated with aCD3/28)
Lane 3 : Human peripheral blood mononuclear cell lysate (treated with IFNγ)
Lane 4 : SOCS3 protein
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG
at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 24.7 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 15 minutes
Protocols
References
This product has been referenced in:
- Gallaher ZR & Steward O Modest enhancement of sensory axon regeneration in the sciatic nerve with conditional co-deletion of PTEN and SOCS3 in the dorsal root ganglia of adult mice. Exp Neurol 303:120-133 (2018). WB . Read more (PubMed: 29458059) »
- Tsai CC et al. Melatonin alleviates liver steatosis induced by prenatal dexamethasone exposure and postnatal high-fat diet. Exp Ther Med 16:917-924 (2018). Read more (PubMed: 30112044) »
