Key features and details
- Mouse monoclonal [OTI3D3] to SOCS3
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-SOCS3 antibody [OTI3D3]
See all SOCS3 primary antibodies
DescriptionMouse monoclonal [OTI3D3] to SOCS3
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
Recombinant full length protein corresponding to Human SOCS3 aa 1-225. Produced in HEK-293T cells (NP_003946).
MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESGFYWSA VTGGEANLLLSAEPAGTFLIRDSSDQRHFFTLSVKTQSGTKNLRIQCEGG SFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPPTEPSSEVPEQ PSAQPLPGSPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGH LDSYEKVTQLPGPIREFLDQYDAPL
Database link: O14543
- WB: pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate. IHC-P: Human colon adenocarcinoma and thyroid carcinoma tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA. Flow: HeLa and Jurkat cells.
Clone OTI3D3 (formerly 3D3).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 24 kDa.|
Perform heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.
FunctionSOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
Tissue specificityWidely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
PathwayProtein modification; protein ubiquitination.
Involvement in diseaseNote=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
Sequence similaritiesContains 1 SH2 domain.
Contains 1 SOCS box domain.
DomainThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
modificationsPhosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
- Information by UniProt
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
All lanes : Anti-SOCS3 antibody [OTI3D3] (ab236519) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY SOCS3 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa
Paraffin-embedded human colon adenocarcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human thyroid carcinoma tissue stained for SOCS3 using ab236519 at 1/150 dilution in immunohistochemical analysis.
COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY SOCS3 cDNA stained for SOCS3 (green) using ab236519 at 1/100 dilution in ICC/IF.
Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).
Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cell line labeling SOCS3 with ab236519 at 1/100 dilution (red) compared to a non-specific negative control antibody (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236519 has not yet been referenced specifically in any publications.